BMRB Entry 18353

Name: 1H, 15N and 13C backbone resonance assignments of the Kelch domain of mouse Keap1
Published: 2012-06-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18353

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 15N and 13C backbone resonance assignments of the Kelch domain of mouse Keap1 PubMed: 22688683

Deposition date: 2012-03-27 Original release date: 2012-06-11

Authors: Cino, Elio; Choy, Wing-Yiu; Fan, Jingsong; Yang, Daiwen

Citation: Cino, Elio; Fan, Jingsong; Yang, Daiwen; Choy, Wing-Yiu. "1H, 15N and 13C backbone resonance assignments of the Kelch domain of mouse Keap1." Biomol. NMR Assignments 7, 149-153 (2013).

Assembly members:
Kelch_domain_of_mouse_Keap1, polymer, 290 residues, Formula weight is not available

Natural source: Common Name: House Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Kelch_domain_of_mouse_Keap1: GVGRLIYTAGGYFRQSLSYL EAYNPSNGSWLRLADLQVPR SGLAGCVVGGLLYAVGGRNN SPDGNTDSSALDCYNPMTNQ WSPCASMSVPRNRIGVGVID GHIYAVGGSHGCIHHSSVER YEPERDEWHLVAPMLTRRIG VGVAVLNRLLYAVGGFDGTN RLNSAECYYPERNEWRMITP MNTIRSGAGVCVLHNCIYAA GGYDGQDQLNSVERYDVETE TWTFVAPMRHHRSALGITVH QGKIYVLGGYDGHTFLDSVE CYDPDSDTWSEVTRMTSGRS GVGVAVTMEP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	490
15N chemical shifts	251
1H chemical shifts	251

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Kelch domain of mouse Keap1	1

Entities:

Entity 1, Kelch domain of mouse Keap1 290 residues - Formula weight is not available

The starting G was a non-native residue from the TEV recognition sequence that remained after cleavage

1	GLY	VAL	GLY	ARG	LEU	ILE	TYR	THR	ALA	GLY
2	GLY	TYR	PHE	ARG	GLN	SER	LEU	SER	TYR	LEU
3	GLU	ALA	TYR	ASN	PRO	SER	ASN	GLY	SER	TRP
4	LEU	ARG	LEU	ALA	ASP	LEU	GLN	VAL	PRO	ARG
5	SER	GLY	LEU	ALA	GLY	CYS	VAL	VAL	GLY	GLY
6	LEU	LEU	TYR	ALA	VAL	GLY	GLY	ARG	ASN	ASN
7	SER	PRO	ASP	GLY	ASN	THR	ASP	SER	SER	ALA
8	LEU	ASP	CYS	TYR	ASN	PRO	MET	THR	ASN	GLN
9	TRP	SER	PRO	CYS	ALA	SER	MET	SER	VAL	PRO
10	ARG	ASN	ARG	ILE	GLY	VAL	GLY	VAL	ILE	ASP
11	GLY	HIS	ILE	TYR	ALA	VAL	GLY	GLY	SER	HIS
12	GLY	CYS	ILE	HIS	HIS	SER	SER	VAL	GLU	ARG
13	TYR	GLU	PRO	GLU	ARG	ASP	GLU	TRP	HIS	LEU
14	VAL	ALA	PRO	MET	LEU	THR	ARG	ARG	ILE	GLY
15	VAL	GLY	VAL	ALA	VAL	LEU	ASN	ARG	LEU	LEU
16	TYR	ALA	VAL	GLY	GLY	PHE	ASP	GLY	THR	ASN
17	ARG	LEU	ASN	SER	ALA	GLU	CYS	TYR	TYR	PRO
18	GLU	ARG	ASN	GLU	TRP	ARG	MET	ILE	THR	PRO
19	MET	ASN	THR	ILE	ARG	SER	GLY	ALA	GLY	VAL
20	CYS	VAL	LEU	HIS	ASN	CYS	ILE	TYR	ALA	ALA
21	GLY	GLY	TYR	ASP	GLY	GLN	ASP	GLN	LEU	ASN
22	SER	VAL	GLU	ARG	TYR	ASP	VAL	GLU	THR	GLU
23	THR	TRP	THR	PHE	VAL	ALA	PRO	MET	ARG	HIS
24	HIS	ARG	SER	ALA	LEU	GLY	ILE	THR	VAL	HIS
25	GLN	GLY	LYS	ILE	TYR	VAL	LEU	GLY	GLY	TYR
26	ASP	GLY	HIS	THR	PHE	LEU	ASP	SER	VAL	GLU
27	CYS	TYR	ASP	PRO	ASP	SER	ASP	THR	TRP	SER
28	GLU	VAL	THR	ARG	MET	THR	SER	GLY	ARG	SER
29	GLY	VAL	GLY	VAL	ALA	VAL	THR	MET	GLU	PRO

Samples:

sample_1: Kelch domain, [U-13C; U-15N; U-2H], 0.2 – 0.3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

SPARKY, Goddard - data analysis

NMR spectrometers:

Varian INOVA 800 MHz
Bruker Avance 800 MHz

PDB	1X2J 1X2R 2DYH 2Z32 3ADE 3WDZ 3WN7 5CGJ
DBJ	BAA34639 BAB23519 BAC32621 BAC36267 BAC97871
EMBL	CAG15151
GB	AAH55732 AAI51546 AAL84711 EDL25167 EDL25168
REF	NP_001094612 NP_001103775 NP_001103776 NP_001103777 NP_001108143
SP	Q684M4 Q9Z2X8
TPG	DAA27941

Biological Magnetic Resonance Data Bank