BMRB Entry 18356
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18356
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution Structure of ADF like UNC-60A Protein of Caenorhabditis elegans
Deposition date: 2012-03-28 Original release date: 2013-05-20
Authors: Shukla, Vaibhav Kumar; Yadav, Rahul; Kabra, Ashish; Kumar, Dinesh; Ono, Shoichiro; Arora, Ashish
Citation: Shukla, Vaibhav Kumar; Yadav, Rahul; Jain, Anupam; Tripathi, Sarita; Kabra, Ashish; Kumar, Dinesh; Ono, Shoichiro; Arora, Ashish. "NMR Structure and Backbone dynamics of ADF like UNC-60A protein from Caenorhabditis elegans: its divergence from conventional ADF/cofilin" J. Biol. Chem. ., .-..
Assembly members:
entity, polymer, 165 residues, 18391.953 Da.
Natural source: Common Name: Caenorhabditis elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MSSGVMVDPDVQTSFQKLSE
GRKEYRYIIFKIDENKVIVE
AAVTQDQLGITGDDYDDSSK
AAFDKFVEDVKSRTDNLTDC
RYAVFDFKFTCSRVGAGTSK
MDKIIFLQICPDGASIKKKM
VYASSAAAIKTSLGTGKILQ
FQVSDESEMSHKELLNKLGE
KYGDH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 719 |
| 15N chemical shifts | 162 |
| 1H chemical shifts | 1114 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ADF like UNC-60A Protein | 1 |
Entities:
Entity 1, ADF like UNC-60A Protein 165 residues - 18391.953 Da.
| 1 | MET | SER | SER | GLY | VAL | MET | VAL | ASP | PRO | ASP | ||||
| 2 | VAL | GLN | THR | SER | PHE | GLN | LYS | LEU | SER | GLU | ||||
| 3 | GLY | ARG | LYS | GLU | TYR | ARG | TYR | ILE | ILE | PHE | ||||
| 4 | LYS | ILE | ASP | GLU | ASN | LYS | VAL | ILE | VAL | GLU | ||||
| 5 | ALA | ALA | VAL | THR | GLN | ASP | GLN | LEU | GLY | ILE | ||||
| 6 | THR | GLY | ASP | ASP | TYR | ASP | ASP | SER | SER | LYS | ||||
| 7 | ALA | ALA | PHE | ASP | LYS | PHE | VAL | GLU | ASP | VAL | ||||
| 8 | LYS | SER | ARG | THR | ASP | ASN | LEU | THR | ASP | CYS | ||||
| 9 | ARG | TYR | ALA | VAL | PHE | ASP | PHE | LYS | PHE | THR | ||||
| 10 | CYS | SER | ARG | VAL | GLY | ALA | GLY | THR | SER | LYS | ||||
| 11 | MET | ASP | LYS | ILE | ILE | PHE | LEU | GLN | ILE | CYS | ||||
| 12 | PRO | ASP | GLY | ALA | SER | ILE | LYS | LYS | LYS | MET | ||||
| 13 | VAL | TYR | ALA | SER | SER | ALA | ALA | ALA | ILE | LYS | ||||
| 14 | THR | SER | LEU | GLY | THR | GLY | LYS | ILE | LEU | GLN | ||||
| 15 | PHE | GLN | VAL | SER | ASP | GLU | SER | GLU | MET | SER | ||||
| 16 | HIS | LYS | GLU | LEU | LEU | ASN | LYS | LEU | GLY | GLU | ||||
| 17 | LYS | TYR | GLY | ASP | HIS |
Samples:
sample_1: UNC-60A, [U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O, [U-99% 2H], 7%; H2O 93%
sample_2: UNC-60A, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O, [U-99% 2H], 7%; H2O 93%
sample_3: UNC-60A, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O 100%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_3 | isotropic | sample_conditions_1 |
| 2D CB(CGCDCE)HE | sample_2 | isotropic | sample_conditions_1 |
| 2D CB(CGCD)HD | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - data analysis
PSVS v1.4, Bhattacharya and Montelione - Validation of structural parameter
iCING, Doreleijers - Validation of structural parameter
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - Structure Calculation
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance3 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts