BMRB Entry 18412
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18412
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: High Definition Solution Structure of PED/PEA-15 Death Effector Domain PubMed: 22732408
Deposition date: 2012-04-20 Original release date: 2012-08-06
Authors: Twomey, Edward; Wei, Yufeng
Citation: Twomey, Edward; Wei, Yufeng. "High-definition NMR structure of PED/PEA-15 death effector domain reveals details of key polar side chain interactions" Biochem. Biophys. Res. Commun. 424, 141-146 (2012).
Assembly members:
pea-15, polymer, 130 residues, 10450.842 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
pea-15: MAEYGTLLQDLTNNITLEDL
EQLKSACKEDIPSEKSEEIT
TGSAWFSFLESHNKLDKDNL
SYIEHIFEISRRPDLLTMVV
DYRTRVLKISEEDELDTKLT
RIPSAKKYKDIIRQPSEEEI
IKLAPPPKKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 472 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 858 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | phosphoprotein enriched in astrocytes 15A | 1 |
Entities:
Entity 1, phosphoprotein enriched in astrocytes 15A 130 residues - 10450.842 Da.
| 1 | MET | ALA | GLU | TYR | GLY | THR | LEU | LEU | GLN | ASP | |
| 2 | LEU | THR | ASN | ASN | ILE | THR | LEU | GLU | ASP | LEU | |
| 3 | GLU | GLN | LEU | LYS | SER | ALA | CYS | LYS | GLU | ASP | |
| 4 | ILE | PRO | SER | GLU | LYS | SER | GLU | GLU | ILE | THR | |
| 5 | THR | GLY | SER | ALA | TRP | PHE | SER | PHE | LEU | GLU | |
| 6 | SER | HIS | ASN | LYS | LEU | ASP | LYS | ASP | ASN | LEU | |
| 7 | SER | TYR | ILE | GLU | HIS | ILE | PHE | GLU | ILE | SER | |
| 8 | ARG | ARG | PRO | ASP | LEU | LEU | THR | MET | VAL | VAL | |
| 9 | ASP | TYR | ARG | THR | ARG | VAL | LEU | LYS | ILE | SER | |
| 10 | GLU | GLU | ASP | GLU | LEU | ASP | THR | LYS | LEU | THR | |
| 11 | ARG | ILE | PRO | SER | ALA | LYS | LYS | TYR | LYS | ASP | |
| 12 | ILE | ILE | ARG | GLN | PRO | SER | GLU | GLU | GLU | ILE | |
| 13 | ILE | LYS | LEU | ALA | PRO | PRO | PRO | LYS | LYS | ALA |
Samples:
sample_1: phosphoprotein enriched in astrocytes 15A, [U-100% 13C; U-100% 15N], 1 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.29, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAC40734 BAE31752 BAE32041 BAE36504 BAE36662 |
| EMBL | CAA60387 CAA60499 CAA74076 CAB51573 CAG46533 |
| GB | AAC97496 AAD56775 AAH02426 AAH22554 AAH38282 |
| REF | NP_001013249 NP_001068924 NP_001127112 NP_001185596 NP_001231780 |
| SP | Q15121 Q5R529 Q5U318 Q62048 Q9Z297 |
| TPG | DAA31971 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts