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Biological Magnetic Resonance Data Bank


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BMRB Entry 18434

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18434
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Title: C-terminal domain of human REV1 in complex with DNA-polymerase H (eta)   PubMed: 22691049

Deposition date: 2012-05-01 Original release date: 2012-06-18

Authors: Pozhidaeva, Alexandra; Pustovalova, Yulia; Pustovalova, Irina; Korzhnev, Dmitry

Citation: Pozhidaeva, Alexandra; Pustovalova, Yulia; Bezsonova, Sanjay; Walker, Irina; Korzhnev, Graham. "NMR structure and dynamics of the C-terminal domain from human Rev1 and its complex with Rev1 interacting region of DNA polymerase ."  Biochemistry 51, 5506-5520 (2012).

Assembly members:
entity_1, polymer, 95 residues, 11011.758 Da.
entity_2, polymer, 16 residues, 1826.117 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GNLAGAVEFNDVKTLLREWI TTISDPMEEDILQVVKYCTD LIEEKDLEKLDLVIKYMKRL MQQSVESVWNMAFDFILDNV QVVLQQTYGSTLKVT
entity_2: QSTGTEPFFKQKSLLL

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts97
1H chemical shifts639

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of human REV11
2DNA-polymerase H (eta)2

Entities:

Entity 1, C-terminal domain of human REV1 95 residues - 11011.758 Da.

1   GLYASNLEUALAGLYALAVALGLUPHEASN
2   ASPVALLYSTHRLEULEUARGGLUTRPILE
3   THRTHRILESERASPPROMETGLUGLUASP
4   ILELEUGLNVALVALLYSTYRCYSTHRASP
5   LEUILEGLUGLULYSASPLEUGLULYSLEU
6   ASPLEUVALILELYSTYRMETLYSARGLEU
7   METGLNGLNSERVALGLUSERVALTRPASN
8   METALAPHEASPPHEILELEUASPASNVAL
9   GLNVALVALLEUGLNGLNTHRTYRGLYSER
10   THRLEULYSVALTHR

Entity 2, DNA-polymerase H (eta) 16 residues - 1826.117 Da.

1   GLNSERTHRGLYTHRGLUPROPHEPHELYS
2   GLNLYSSERLEULEULEU

Samples:

sample_1: REV1, [U-99% 13C; U-99% 15N], 0.9 mM; DNA-polymerase 0.9 mM; sodium phosphate 50 mM; sodium chloride 100 mM; EDTA 0.25 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TALOS, Cornilescu, Delaglio and Bax - data analysis

CARA, Kurt W thrich - data analysis

NMR spectrometers:

  • Varian Avance 800 MHz
  • Varian Avance 500 MHz

Related Database Links:

BMRB 18432 18455
PDB
DBJ BAB21441 BAA81666 BAB18601 BAG51237 BAG53738 BAG57675
EMBL CAB38231 CAH93279
GB AAF06731 AAF18986 AAI30412 AAK43708 AAY24314 AAD43810 AAQ81300 ACQ91143 EAX04210 EAX04211
REF NP_001032961 NP_001126930 NP_057400 XP_001160264 XP_001363717 NP_001278898 NP_006493 XP_003833292 XP_004048392 XP_005249243
SP Q5R4N7 Q9UBZ9 Q9Y253

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts