BMRB Entry 18442
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18442
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Title: Solution structure of the R. rickettsii cold shock-like protein PubMed: 23143233
Deposition date: 2012-05-04 Original release date: 2012-06-05
Authors: Veldkamp, C.; Peterson, F.; Gerarden, K.; Fuchs, A.; Koch, J.; Mueller, M.
Citation: Gerarden, Kyle; Fuchs, Andrew; Koch, Jonathan; Mueller, Melissa; Graupner, David; Frost, Justin; Heinen, Caleb; Lackner, Heather; Schoeller, Emily; House, Scott; Peterson, Paul; Veldkamp, Francis. "Solution structure of the cold-shock-like protein from Rickettsia rickettsii." Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68, 1284-1288 (2012).
Assembly members:
cold_shock-like_protein_[Rickettsia_rickettsii_str._'Sheila_Smith']_, polymer, 70 residues, 7781.821 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cold_shock-like_protein_[Rickettsia_rickettsii_str._'Sheila_Smith']_: MATNIVGKVKWYNSTKNFGF
IEQDNGGKDVFVHKSAVDAA
GLHSLEEGQDVIFDLEEKQG
KAYAVNLRIK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 489 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | R. rickettsii cold shock-like protein | 1 |
Entities:
Entity 1, R. rickettsii cold shock-like protein 70 residues - 7781.821 Da.
| 1 | MET | ALA | THR | ASN | ILE | VAL | GLY | LYS | VAL | LYS | |
| 2 | TRP | TYR | ASN | SER | THR | LYS | ASN | PHE | GLY | PHE | |
| 3 | ILE | GLU | GLN | ASP | ASN | GLY | GLY | LYS | ASP | VAL | |
| 4 | PHE | VAL | HIS | LYS | SER | ALA | VAL | ASP | ALA | ALA | |
| 5 | GLY | LEU | HIS | SER | LEU | GLU | GLU | GLY | GLN | ASP | |
| 6 | VAL | ILE | PHE | ASP | LEU | GLU | GLU | LYS | GLN | GLY | |
| 7 | LYS | ALA | TYR | ALA | VAL | ASN | LEU | ARG | ILE | LYS |
Samples:
sample: [U-100% 13C; U-100% 15N] 1.5 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY (AROMATIC) | sample | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement
TOPSPIN v2.1, Bruker - collection
NMRPipe v2007, Delagio,F. et al. - processing
XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis
GARANT v2.1, C. Bartels - data analysis
CYANA v3.1, Guntert, P. - structural calculation
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAK96972 |
| EMBL | CDI29705 CEO18020 |
| GB | AAL03559 AAY61113 ABV76609 ABV85095 ABY72984 |
| REF | WP_004997759 WP_008579233 WP_011270614 WP_014014650 WP_014365526 |
| SP | Q4UMU5 Q92GV1 |
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