BMRB Entry 18449
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18449
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Title: Structure, sulfatide-binding properties, and inhibition of platelet aggregation by a Disabled-2-derived peptide PubMed: 22977233
Deposition date: 2012-05-08 Original release date: 2012-09-17
Authors: Xiao, Shuyan
Citation: Xiao, Shuyan; Charonko, John; Fu, Xiangping; Salmanzadeh, Alireza; Davalos, Rafael; Vlachos, Pavlos; Finkielstein, Carla; Capelluto, Daniel. "Structure, sulfatide binding properties, and inhibition of platelet aggregation by a disabled-2 protein-derived peptide." J. Biol. Chem. 287, 37691-37702 (2012).
Assembly members:
SBM, polymer, 35 residues, 3993.754 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SBM: SKKEKKKGPEKTDEYLLARF
KGDGVKYKAKLIGID
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 101 |
| 15N chemical shifts | 34 |
| 1H chemical shifts | 233 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SBM | 1 |
Entities:
Entity 1, SBM 35 residues - 3993.754 Da.
| 1 | SER | LYS | LYS | GLU | LYS | LYS | LYS | GLY | PRO | GLU | ||||
| 2 | LYS | THR | ASP | GLU | TYR | LEU | LEU | ALA | ARG | PHE | ||||
| 3 | LYS | GLY | ASP | GLY | VAL | LYS | TYR | LYS | ALA | LYS | ||||
| 4 | LEU | ILE | GLY | ILE | ASP |
Samples:
sample_2: DSS, [U-2H], 50 uM; potassium chloride 40 mM; sodium azide 1 mM; citric, [U-99% 2H], 10 mM; SBM 1 mM
sample_1: DSS, [U-2H], 50 uM; potassium chloride 40 mM; sodium azide 1 mM; citric, [U-99% 2H], 10 mM; SBM 1 mM
sample_3: DSS, [U-2H], 50 uM; potassium chloride 40 mM; sodium azide 1 mM; citric, [U-99% 2H], 10 mM; SBM 1 mM
sample_conditions_1: pH: 5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HN(CA)CO | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22405 BAE32784 BAE37047 BAE37084 BAE38234 |
| EMBL | CAH89644 |
| GB | AAA98975 AAB02645 AAB02646 AAB02647 AAB19032 |
| PIR | A57542 |
| REF | NP_001008702 NP_001032994 NP_001095870 NP_001124738 NP_001180175 |
| SP | O88797 P98078 P98082 |
| TPG | DAA17841 |
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