BMRB Entry 18515

Name: high resolution NMR solution structure of helix H1 of the human HAR1 RNA
Published: 2012-07-24

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PDB ID: 2lub
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18515
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: high resolution NMR solution structure of helix H1 of the human HAR1 RNA PubMed: 22961937

Deposition date: 2012-06-11 Original release date: 2012-07-24

Authors: Cevec, Mirko; Ziegeler, Melanie; Richter, Christian; Schwalbe, Harald

Citation: Ziegeler, Melanie; Cevec, Mirko; Richter, Christian; Schwalbe, Harald. "NMR Studies of HAR1 RNA Secondary Structures Reveal Conformational Dynamics in the Human RNA" Chembiochem 13, 2100-2112 (2012).

Assembly members:
RNA_(37-MER), polymer, 37 residues, 11887.177 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
RNA_(37-MER): GGGUGAAACGGAGGACUUCG GUCCUCAAGUUUCACCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	277

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA (37-MER)	1

Entities:

Entity 1, RNA (37-MER) 37 residues - 11887.177 Da.

1

G

U

G

A

C

G

2

G

A

G

A

C

U

C

G

3

G

U

C

U

C

A

G

U

4

U

C

A

C

Samples:

sample_1: RNA (37-MER), [U-100% 15N], 1 mM; potassium chloride 50 mM; potassium phosphate 25 mM; H2O 90%; D2O 10%

sample_2: RNA (37-MER), [U-100% 15N], 1 mM; potassium chloride 50 mM; potassium phosphate 25 mM; D2O 100%

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 75 mM; pH: 6.2; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_2
2D HNN-COSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_2
2D 1H-15N CPMG-NOESY	sample_1	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1

Software:

ARIA v1.2, Linge, O, . - refinement

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY v3.113, Goddard - data analysis

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 800 MHz
Bruker Avance 700 MHz
Bruker Avance 600 MHz