BMRB Entry 18535

Name: SOLUTION STRUCTURE OF DOUBLE-STRANDED RNA BINDING DOMAIN OF S.CEREVISIAE RNASE III (RNT1P)
Published: 2012-08-29

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PDB ID: 2luq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18535
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: SOLUTION STRUCTURE OF DOUBLE-STRANDED RNA BINDING DOMAIN OF S.CEREVISIAE RNASE III (RNT1P) PubMed: 21742266

Deposition date: 2012-06-19 Original release date: 2012-08-29

Authors: Wang, Zhonghua; Feigon, Juli

Citation: Wang, Zhonghua; Hartman, Elon; Roy, Kevin; Chanfreau, Guillaume; Feigon, Juli. "Structure of a yeast RNase III dsRBD complex with a noncanonical RNA substrate provides new insights into binding specificity of dsRBDs." Structure 19, 999-1010 (2011).

Assembly members:
Ribonuclease III, polymer, 90 residues, 9822.442 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Ribonuclease III: GSLDMNAKRQLYSLIGYASL RLHYVTVKKPTAVDPNSIVE CRVGDGTVLGTGVGRNIKIA GIRAAENALRDKKMLDFYAK QRAAIPRSES

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	355
15N chemical shifts	84
1H chemical shifts	539

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ribonuclease III	1

Entities:

Entity 1, Ribonuclease III 90 residues - 9822.442 Da.

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

Samples:

sample_1: Ribonuclease III, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; sodium chloride 150 mM; sodium phosphate 20 mM

sample_2: Ribonuclease III, [U-100% 13C; U-100% 15N], 1 mM; D2O, [U-100% 2H], 100%; sodium chloride 150 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN v2.0, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz

BMRB	17574 18534
PDB	1T4L 1T4N 1T4O 2LBS 2LUP 2LUQ 4OOG
DBJ	GAA25690
EMBL	CAA90210 CAY82070
GB	AAB04172 AHY76694 AJP40933 AJS62105 AJS62540
REF	NP_013966
SP	Q02555
TPG	DAA10139

Biological Magnetic Resonance Data Bank