BMRB Entry 18546
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18546
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Title: Solution structure of the tandem zinc finger domain of fission yeast Stc1 PubMed: 23613586
Deposition date: 2012-06-22 Original release date: 2013-05-06
Authors: He, Chao; Shi, Yun; Bayne, Elizabeth; Wu, Hui
Citation: He, Chao; Pillai, Sreerekha; Taglini, Francesca; Li, Fudong; Ruan, Ke; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu; Bayne, Elizabeth. "Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification." Proc. Natl. Acad. Sci. U.S.A. 110, E1879-E1888 (2013).
Assembly members:
entity_1, polymer, 97 residues, 11085.919 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: HMGKNDNDALIMCMRCRKVK
GIDSYSKTQWSKTFTFVRGR
TVSVSDPKVICRTCQPKQHD
SIWCTACQQTKGINEFSKAQ
RHVLDPRCQICVHSQRN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 385 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 649 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tandem zinc finger domain of Stc1 | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
Entities:
Entity 1, tandem zinc finger domain of Stc1 97 residues - 11085.919 Da.
| 1 | HIS | MET | GLY | LYS | ASN | ASP | ASN | ASP | ALA | LEU | ||||
| 2 | ILE | MET | CYS | MET | ARG | CYS | ARG | LYS | VAL | LYS | ||||
| 3 | GLY | ILE | ASP | SER | TYR | SER | LYS | THR | GLN | TRP | ||||
| 4 | SER | LYS | THR | PHE | THR | PHE | VAL | ARG | GLY | ARG | ||||
| 5 | THR | VAL | SER | VAL | SER | ASP | PRO | LYS | VAL | ILE | ||||
| 6 | CYS | ARG | THR | CYS | GLN | PRO | LYS | GLN | HIS | ASP | ||||
| 7 | SER | ILE | TRP | CYS | THR | ALA | CYS | GLN | GLN | THR | ||||
| 8 | LYS | GLY | ILE | ASN | GLU | PHE | SER | LYS | ALA | GLN | ||||
| 9 | ARG | HIS | VAL | LEU | ASP | PRO | ARG | CYS | GLN | ILE | ||||
| 10 | CYS | VAL | HIS | SER | GLN | ARG | ASN |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: sodium chloride 200 mM; Bis-Tris 20 mM; entity_1, [U-100% 13C; U-100% 15N], 0.8 mM; ZINC ION 1.6 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, peak picking
TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation
Molmol, Koradi, Billeter and Wuthrich - data analysis
ProcheckNMR, Laskowski and MacArthur - data analysis
NMR spectrometers:
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts