BMRB Entry 18586
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18586
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Title: Solution structures of Miz-1 zinc fingers 8 to 10 PubMed: 22986688
Deposition date: 2012-07-10 Original release date: 2013-07-16
Authors: Bedard, Mikael; Maltais, Loika; Beaulieu, Marie-Eve; Bernard, David; Lavigne, Pierre
Citation: Bedard, Mikael; Maltais, Loika; Beaulieu, Marie-Eve; Bilodeau, Josee; Bernard, David; Lavigne, Pierre. "NMR structure note: solution structure of human Miz-1 zinc fingers 8 to 10." J. Biomol. NMR 54, 317-323 (2012).
Assembly members:
Miz8-10, polymer, 83 residues, 9512.0695 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Miz8-10: MKPYVCIHCQRQFADPGALQ
RHVRIHTGEKPCQCVMCGKA
FTQASSLIAHVRQHTGEKPY
VCERCGKRFVQSSQLANHIR
HHD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 316 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 484 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Miz8-10 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Miz8-10 83 residues - 9512.0695 Da.
| 1 | MET | LYS | PRO | TYR | VAL | CYS | ILE | HIS | CYS | GLN | ||||
| 2 | ARG | GLN | PHE | ALA | ASP | PRO | GLY | ALA | LEU | GLN | ||||
| 3 | ARG | HIS | VAL | ARG | ILE | HIS | THR | GLY | GLU | LYS | ||||
| 4 | PRO | CYS | GLN | CYS | VAL | MET | CYS | GLY | LYS | ALA | ||||
| 5 | PHE | THR | GLN | ALA | SER | SER | LEU | ILE | ALA | HIS | ||||
| 6 | VAL | ARG | GLN | HIS | THR | GLY | GLU | LYS | PRO | TYR | ||||
| 7 | VAL | CYS | GLU | ARG | CYS | GLY | LYS | ARG | PHE | VAL | ||||
| 8 | GLN | SER | SER | GLN | LEU | ALA | ASN | HIS | ILE | ARG | ||||
| 9 | HIS | HIS | ASP |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample-Miz8-10: Miz8-10, [U-13C; U-15N], 0.75 – 1.00 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D HNCO | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample-Miz8-10 | isotropic | sample_conditions_1 |
| 3D HNHA | sample-Miz8-10 | isotropic | sample_conditions_1 |
Software:
ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - refinement, structure calculation
CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
ANALYSIS v2.1, CCPN - chemical shift assigment, data analysis
DANGLE v1.1, CCPN - data analysis
NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
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or all simulated shifts