BMRB Entry 18589
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18589
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Title: NMR solution structure of Cbp2 PubMed: 23325851
Deposition date: 2012-07-11 Original release date: 2013-01-16
Authors: Kenchappa, Chandra; Heidarsson, Petur; Garrett, Roger; Poulsen, Flemming
Citation: Kenchappa, Chandra; Heidarsson, Petur; Kragelund, Birthe; Garrett, Roger; Poulsen, Flemming. "Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2." Nucleic Acids Res. 41, 3424-3435 (2013).
Assembly members:
Cbp2, polymer, 105 residues, 12415 Da.
Natural source: Common Name: Hyperthermus butylicus Taxonomy ID: 54248 Superkingdom: Archaea Kingdom: not available Genus/species: Hyperthermus butylicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Cbp2: MPSVNDSLDIVEKLYKDGVP
VKEIAKRSNNSMSTVYKALE
KLEAMGRIKRRKGRYRQHRR
LTEEELATIRELYLKGATVY
EIARQLGRPESTIYYALKKL
GLKLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 395 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 589 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cbp2 | 1 |
Entities:
Entity 1, Cbp2 105 residues - 12415 Da.
| 1 | MET | PRO | SER | VAL | ASN | ASP | SER | LEU | ASP | ILE | ||||
| 2 | VAL | GLU | LYS | LEU | TYR | LYS | ASP | GLY | VAL | PRO | ||||
| 3 | VAL | LYS | GLU | ILE | ALA | LYS | ARG | SER | ASN | ASN | ||||
| 4 | SER | MET | SER | THR | VAL | TYR | LYS | ALA | LEU | GLU | ||||
| 5 | LYS | LEU | GLU | ALA | MET | GLY | ARG | ILE | LYS | ARG | ||||
| 6 | ARG | LYS | GLY | ARG | TYR | ARG | GLN | HIS | ARG | ARG | ||||
| 7 | LEU | THR | GLU | GLU | GLU | LEU | ALA | THR | ILE | ARG | ||||
| 8 | GLU | LEU | TYR | LEU | LYS | GLY | ALA | THR | VAL | TYR | ||||
| 9 | GLU | ILE | ALA | ARG | GLN | LEU | GLY | ARG | PRO | GLU | ||||
| 10 | SER | THR | ILE | TYR | TYR | ALA | LEU | LYS | LYS | LEU | ||||
| 11 | GLY | LEU | LYS | LEU | GLU |
Samples:
sample_1: Cbp2, [U-100% 13C; U-100% 15N], 0.5-1 mM; potassium chloride, none, 10 mM; potassium dihydrogen phosphate, none, 20 mM; sodium azide, none, 0.02%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
VNMRJ, Varian - collection
CCPN_Analysis, CCPN - chemical shift assignment, peak picking
PSVS, Bhattacharya and Montelione - data analysis
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts