BMRB Entry 18648
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18648
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: APPTM V44M PubMed: 24390130
Deposition date: 2012-08-07 Original release date: 2012-09-07
Authors: Chen, Wen; Wang, Chunyu
Citation: Chen, Wen; Gamache, Eric; Rosenman, David; Xie, Jian; Lopez, Maria; Li, Yue-Ming; Wang, Chunyu. "Familial Alzheimer's mutations within APPTM increase A42 production by enhancing accessibility of -cleavage site." Nat. Commun. 5, 3037-3037 (2014).
Assembly members:
V44M monomer, polymer, 32 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
V44M monomer: GAMAKGAIIGLMVGGVVIAT
MIVITLVMLKKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 144 |
| 15N chemical shifts | 30 |
| 1H chemical shifts | 252 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | V44M chain, 1 | 1 |
| 2 | V44M chain, 2 | 1 |
Entities:
Entity 1, V44M chain, 1 32 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | LYS | GLY | ALA | ILE | ILE | GLY | ||||
| 2 | LEU | MET | VAL | GLY | GLY | VAL | VAL | ILE | ALA | THR | ||||
| 3 | MET | ILE | VAL | ILE | THR | LEU | VAL | MET | LEU | LYS | ||||
| 4 | LYS | LYS |
Samples:
15N_V44M: APPTM V44M dimer, [U-100% 13C; U-100% 15N], mM; potassium chloride 0.5 mM; sodium chloride 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 15N_V44M | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | 15N_V44M | isotropic | sample_conditions_1 |
| 3D C(CO)NH | 15N_V44M | isotropic | sample_conditions_1 |
| 3D HNCO | 15N_V44M | isotropic | sample_conditions_1 |
| 3D HNCACB | 15N_V44M | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | 15N_V44M | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | 15N_V44M | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | 15N_V44M | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | 15N_V44M | isotropic | sample_conditions_1 |
Software:
SPARKY, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts