BMRB Entry 18658
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18658
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Title: NMR solution structure of peptide a2N(1-17) from Mus musculus V-ATPase PubMed: 23288846
Deposition date: 2012-08-14 Original release date: 2013-01-18
Authors: Dip, Phat Vinh; Gruber, Gerhard; Marshansky, Vladimir
Citation: Hosokawa, Hiroyuki; Dip, Phat Vinh; Merkulova, Maria; Bakulina, Anastasia; Zhuang, Zhenjie; Khatri, Ashok; Jian, Xiaoying; Keating, Shawn; Bueler, Stephanie; Rubinstein, John; Randazzo, Paul; Ausiello, Dennis; Gruber, Gerhard; Marshansky, Vladimir. "The N Termini of a-Subunit Isoforms Are Involved in Signaling between Vacuolar H+-ATPase (V-ATPase) and Cytohesin-2." J. Biol. Chem. 288, 5896-5913 (2013).
Assembly members:
a2N(1-17), polymer, 17 residues, 1934.332 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
a2N(1-17): MGSLFRSESMCLAQLFL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | peptide a2N(1-17) from Mus musculus V-ATPase | 1 |
Entities:
Entity 1, peptide a2N(1-17) from Mus musculus V-ATPase 17 residues - 1934.332 Da.
| 1 | MET | GLY | SER | LEU | PHE | ARG | SER | GLU | SER | MET | ||||
| 2 | CYS | LEU | ALA | GLN | LEU | PHE | LEU |
Samples:
sample_1: a2N(1-17) 2 mM; TFE, [U-99% 2H], 50%; sodium phosphate 25 mM; sodium chloride 300 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis, peak picking
TALOS, Cornilescu, Delaglio and Bax - geometry optimization
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
NMR spectrometers:
- Bruker Avance 600 MHz