BMRB Entry 18698
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18698
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Title: Solution NMR Structure of the eukaryotic RNA recognition motif, RRM1, from the heterogeneous nuclear ribonucleoprotein H from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8614A
Deposition date: 2012-08-31 Original release date: 2012-10-29
Authors: Ramelot, Theresa; Yang, Yunhuang; Pederson, Kari; Shastry, Ritu; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Prestegard, James; Montelione, Gaetano; Kennedy, Michael
Citation: Ramelot, Theresa; Yang, Yunhuang; Pederson, Kari; Shastry, Ritu; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Prestegard, James; Montelione, Gaetano; Kennedy, Michael. "Solution NMR Structure of the eukaryotic RNA recognition motif, RRM1, from the heterogeneous nuclear ribonucleoprotein H from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8614A" To be published ., .-..
Assembly members:
HR8614A, polymer, 108 residues, 12381.880 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HR8614A: SHMGGEGFVVKVRGLPWSCS
ADEVQRFFSDCKIQNGAQGI
RFIYTREGRPSGEAFVELES
EDEVKLALKKDRETMGHRYV
EVFKSNNVEMDWVLKHTGPN
SPDTANDG
- assigned_chemical_shifts
- conformer_family_coord_set
- representative_conformer
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 466 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 723 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HR8614A | 1 |
Entities:
Entity 1, HR8614A 108 residues - 12381.880 Da.
| 1 | SER | HIS | MET | GLY | GLY | GLU | GLY | PHE | VAL | VAL | ||||
| 2 | LYS | VAL | ARG | GLY | LEU | PRO | TRP | SER | CYS | SER | ||||
| 3 | ALA | ASP | GLU | VAL | GLN | ARG | PHE | PHE | SER | ASP | ||||
| 4 | CYS | LYS | ILE | GLN | ASN | GLY | ALA | GLN | GLY | ILE | ||||
| 5 | ARG | PHE | ILE | TYR | THR | ARG | GLU | GLY | ARG | PRO | ||||
| 6 | SER | GLY | GLU | ALA | PHE | VAL | GLU | LEU | GLU | SER | ||||
| 7 | GLU | ASP | GLU | VAL | LYS | LEU | ALA | LEU | LYS | LYS | ||||
| 8 | ASP | ARG | GLU | THR | MET | GLY | HIS | ARG | TYR | VAL | ||||
| 9 | GLU | VAL | PHE | LYS | SER | ASN | ASN | VAL | GLU | MET | ||||
| 10 | ASP | TRP | VAL | LEU | LYS | HIS | THR | GLY | PRO | ASN | ||||
| 11 | SER | PRO | ASP | THR | ALA | ASN | ASP | GLY |
Samples:
HR8614A.007_NC_sample: HR8614A.007, [U-100% 13C; U-100% 15N], 1.0 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM
HR8614A.009_NC5_sample: HR8614A.008, [biosynthetically directed-5% 13C; U-100% 15N], 0.8 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM
HR8614A.007_NC_D2O_sample: HR8614A.007, [U-100% 13C; U-100% 15N], 1.0 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D HNCO | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D HNCACB | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D 1H-13C arom NOESY | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D C(CO)NH | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | HR8614A.007_NC_D2O_sample | isotropic | sample_conditions_1 |
| 3D NUS 1H-13C NOESY aliphatic | HR8614A.007_NC_D2O_sample | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC NH2only | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 4D 13C-13C-HMQC-NOESY-HMQC | HR8614A.007_NC_D2O_sample | isotropic | sample_conditions_1 |
| 1D T1 Nhsqc array | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 1D T2 Nhsqc array | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | HR8614A.009_NC5_sample | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | HR8614A.009_NC5_sample | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC arom CT | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC arom noCT | HR8614A.007_NC_sample | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC_His | HR8614A.009_NC5_sample | isotropic | sample_conditions_1 |
| 2D 1H-15N heteronuclear NOE interleaved | HR8614A.009_NC5_sample | isotropic | sample_conditions_1 |
Software:
CNS vcns_solve_1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution
AutoStruct vASDP-1.0, Huang, Tejero, Powers and Montelione - data analysis,refinement
NMRPipe vNMRPipe-201109, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN v2.1.4 and 3.1, Bruker Biospin - collection
VNMRJ v1.1 D, Varian - collection
PINE vPINE Server v.2.0, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
SPARKY v3.113, Goddard - data analysis
TALOS+ v1.2009.0721.18, Shen, Cornilescu, Delaglio and Bax - geometry optimization
PALES vpales_linux 2000, PALES (Zweckstetter, Bax) - geometry optimization
PSVS v1.4, Bhattacharya, Montelione - structure validation
FMCGUI vfmcgui_2.5_linux, Lemak, Gutmanas, Chitayat, Karra, Fares, Sunnerhagan, and Arrowsmith - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts