BMRB Entry 18702
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18702
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence. PubMed: 23142982
Deposition date: 2012-09-05 Original release date: 2012-11-05
Authors: Nicastro, Giuseppe; Garcia-mayoral, M.; Hollingworth, D.; Kelly, G.; Martin, S.; Briata, P.; Gherzi, R.; Ramos, A.
Citation: Nicastro, Giuseppe; Garcia-Mayoral, Maria Flor; Hollingworth, David; Kelly, Geoff; Martin, Stephen; Briata, Paola; Gherzi, Roberto; Ramos, Andres. "Noncanonical G recognition mediates KSRP regulation of let-7 biogenesis." Nat. Struct. Mol. Biol. 19, 1282-1286 (2012).
Assembly members:
KH-TYPE_SPLICING_REGULATORY_PROTEIN, polymer, 106 residues, 10903.4744 Da.
5'-R(*AP*GP*GP*GP*UP)-3', polymer, 5 residues, 1690.0234 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
KH-TYPE_SPLICING_REGULATORY_PROTEIN: GAMAYGSRIGGGIDVPVPRH
SVGVVIGRSGEMIKKIQNDA
GVRIQFKQDDGTGPEKIAHI
MGPPDRCEHAARIINDLLQS
LRSGPPGPPGGPGMPPGGRG
RGRGQG
5'-R(*AP*GP*GP*GP*UP)-3': AGGGU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 235 |
| 1H chemical shifts | 523 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KH-TYPE SPLICING REGULATORY PROTEIN | 1 |
| 2 | 5'-R(*AP*GP*GP*GP*UP)-3' | 2 |
Entities:
Entity 1, KH-TYPE SPLICING REGULATORY PROTEIN 106 residues - 10903.4744 Da.
| 1 | GLY | ALA | MET | ALA | TYR | GLY | SER | ARG | ILE | GLY | ||||
| 2 | GLY | GLY | ILE | ASP | VAL | PRO | VAL | PRO | ARG | HIS | ||||
| 3 | SER | VAL | GLY | VAL | VAL | ILE | GLY | ARG | SER | GLY | ||||
| 4 | GLU | MET | ILE | LYS | LYS | ILE | GLN | ASN | ASP | ALA | ||||
| 5 | GLY | VAL | ARG | ILE | GLN | PHE | LYS | GLN | ASP | ASP | ||||
| 6 | GLY | THR | GLY | PRO | GLU | LYS | ILE | ALA | HIS | ILE | ||||
| 7 | MET | GLY | PRO | PRO | ASP | ARG | CYS | GLU | HIS | ALA | ||||
| 8 | ALA | ARG | ILE | ILE | ASN | ASP | LEU | LEU | GLN | SER | ||||
| 9 | LEU | ARG | SER | GLY | PRO | PRO | GLY | PRO | PRO | GLY | ||||
| 10 | GLY | PRO | GLY | MET | PRO | PRO | GLY | GLY | ARG | GLY | ||||
| 11 | ARG | GLY | ARG | GLY | GLN | GLY |
Entity 2, 5'-R(*AP*GP*GP*GP*UP)-3' 5 residues - 1690.0234 Da.
| 1 | A | G | G | G | U |
Samples:
sample_1: KH-TYPE SPLICING REGULATORY PROTEIN, [U-13C; U-15N], 300 uM; 5'-R(*AP*GP*GP*GP*UP)-3', [U-13C; U-15N], 300 uM
sample_conditions_1: ionic strength: 100.000 mM; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Experiment-1 | sample_1 | solution | sample_conditions_1 |
Software:
ARIA v1.2, Linge, O, . - chemical shift assignment
AutoDep v4.3, PDBe - chemical shift assignment
CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - data analysis
NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment
SPARKY vany, Goddard - chemical shift assignment
UXNMR v3.5, Bruker Biospin - chemical shift assignment
VNMR vany, Varian - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz