BMRB Entry 18813
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18813
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Title: The solution structure of human PHF1 in complex with H3K36me3 PubMed: 23273982
Deposition date: 2012-10-30 Original release date: 2012-01-15
Authors: Song, Jikui; Patel, Dinshaw
Citation: Cai, Ling; Rothbart, Scott; Lu, Rui; Xu, Bowen; Chen, Wei-Yi; Tripathy, Ashutosh; Rockowitz, Shira; Zheng, Deyou; Patel, Dinshaw; Allis, C. David; Strahl, Brian; Song, Jikui; Wang, Gang Greg. "An H3K36 Methylation-Engaging Tudor Motif of Polycomb-like Proteins Mediates PRC2 Complex Targeting." Mol. Cell 49, 571-582 (2013).
Assembly members:
entity_1, polymer, 79 residues, 8737.930 Da.
entity_2, polymer, 11 residues, 1259.513 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: SRLSRSGASSLWDPASPAPT
SGPRPRLWEGQDVLARWTDG
LLYLGTIKKVDSAREVCLVQ
FEDDSQFLVLWKDISPAAL
entity_2: ATGGVXKPHRY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 239 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 428 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human PHF1 | 1 |
| 2 | H3K36me3 | 2 |
Entities:
Entity 1, human PHF1 79 residues - 8737.930 Da.
| 1 | SER | ARG | LEU | SER | ARG | SER | GLY | ALA | SER | SER | ||||
| 2 | LEU | TRP | ASP | PRO | ALA | SER | PRO | ALA | PRO | THR | ||||
| 3 | SER | GLY | PRO | ARG | PRO | ARG | LEU | TRP | GLU | GLY | ||||
| 4 | GLN | ASP | VAL | LEU | ALA | ARG | TRP | THR | ASP | GLY | ||||
| 5 | LEU | LEU | TYR | LEU | GLY | THR | ILE | LYS | LYS | VAL | ||||
| 6 | ASP | SER | ALA | ARG | GLU | VAL | CYS | LEU | VAL | GLN | ||||
| 7 | PHE | GLU | ASP | ASP | SER | GLN | PHE | LEU | VAL | LEU | ||||
| 8 | TRP | LYS | ASP | ILE | SER | PRO | ALA | ALA | LEU |
Entity 2, H3K36me3 11 residues - 1259.513 Da.
| 1 | ALA | THR | GLY | GLY | VAL | M3L | LYS | PRO | HIS | ARG | ||||
| 2 | TYR |
Samples:
sample_1: human PHF1, [U-100% 13C; U-100% 15N], 0.3 – 0.5 mM; H3K36me30.6 – 1.0 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker DMX 900 MHz
- Bruker DMX 700 MHz
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA25074 BAE00455 BAG58363 BAH14870 BAK62078 |
| EMBL | CAA16158 CAA16159 CAC38366 CAC38367 CAE83917 |
| GB | AAC13273 AAC52062 AAD00518 AAH08834 AAH83843 |
| REF | NP_001092384 NP_001244355 NP_001267246 NP_001289326 NP_002627 |
| SP | O43189 Q9Z1B8 |
| TPG | DAA16680 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts