BMRB Entry 18822
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18822
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Title: Solution Structure of the trans-membrane domain of the NS2A of dengue virus PubMed: 23408612
Deposition date: 2012-11-05 Original release date: 2013-02-21
Authors: Gayen, Shovanlal; Kang, Congbao
Citation: Xie, Xuping; Gayen, Shovanlal; Kang, Congbao; Yuan, Zhiming; Shi, Pei-Yong. "Membrane Topology and Function of Dengue Virus NS2A Protein." J. Virol. 87, 4609-4622 (2013).
Assembly members:
entity, polymer, 28 residues, 2981.647 Da.
Natural source: Common Name: Dengue virus Taxonomy ID: 12637 Superkingdom: Viruses Kingdom: not available Genus/species: Flavivirus Dengue virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: DIGMGVTYLALLAAFKVRPT
FAAGLLLR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 26 |
| 15N chemical shifts | 26 |
| 1H chemical shifts | 179 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | trans-membrane domain of the NS2A | 1 |
Entities:
Entity 1, trans-membrane domain of the NS2A 28 residues - 2981.647 Da.
| 1 | ASP | ILE | GLY | MET | GLY | VAL | THR | TYR | LEU | ALA | ||||
| 2 | LEU | LEU | ALA | ALA | PHE | LYS | VAL | ARG | PRO | THR | ||||
| 3 | PHE | ALA | ALA | GLY | LEU | LEU | LEU | ARG |
Samples:
sample_1: entity 3 mg/mL; chloroform, [U-2H], 44.4%; methanol, [U-2H], 44.4%; H2O 11.2%
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAD36758 BAD36759 BAD36760 BAD42415 BAD42416 |
| EMBL | CAD31751 CAI92123 CAR65133 CAR65134 CAR65135 |
| GB | AAA42941 AAA42942 AAA42962 AAA66406 AAA73185 |
| PRF | 1301272A |
| REF | NP_056776 NP_739585 |
| SP | P07564 P12823 P14337 P14340 P29990 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts