BMRB Entry 18880
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18880
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Title: SOLUTION STRUCTURE OF THE SMALL DICTYOSTELIUM DISCOIDEIUM MYOSIN LIGHT CHAIN MlcB PROVIDES INSIGHTS INTO IQ-MOTIF RECOGNITION OF CLASS I MYOSIN MYO1B PubMed: 24790102
Deposition date: 2012-12-06 Original release date: 2013-12-09
Authors: Liburd, Janine; Chitayat, Seth; Crawley, Scott; Denis, Christopher; Cote, Graham; Smith, Steven
Citation: Liburd, Janine; Chitayat, Seth; Crawley, Scott; Munro, Kim; Miller, Emily; Denis, Chris; Spencer, Holly; Cote, Graham; Smith, Steven. "Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ motif Recognition." J. Biol. Chem. ., .-. (2014).
Assembly members:
MlcB, polymer, 93 residues, 10471.464 Da.
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MlcB: MGSSHHHHHHSSGLVPRGSH
MSDEKTQLIEAFYNFDGDYD
GFVSVEEFRGIIRDGLPMTE
AEITEFFEAADPNNTGFIDY
KAFAAMLYSVDES
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 322 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 519 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MlcB | 1 |
Entities:
Entity 1, MlcB 93 residues - 10471.464 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | SER | ASP | GLU | LYS | THR | GLN | LEU | ILE | GLU | ||||
| 4 | ALA | PHE | TYR | ASN | PHE | ASP | GLY | ASP | TYR | ASP | ||||
| 5 | GLY | PHE | VAL | SER | VAL | GLU | GLU | PHE | ARG | GLY | ||||
| 6 | ILE | ILE | ARG | ASP | GLY | LEU | PRO | MET | THR | GLU | ||||
| 7 | ALA | GLU | ILE | THR | GLU | PHE | PHE | GLU | ALA | ALA | ||||
| 8 | ASP | PRO | ASN | ASN | THR | GLY | PHE | ILE | ASP | TYR | ||||
| 9 | LYS | ALA | PHE | ALA | ALA | MET | LEU | TYR | SER | VAL | ||||
| 10 | ASP | GLU | SER |
Samples:
sample_1: MlcB, [U-100% 13C; U-100% 15N], 1 mM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.5 mM; H2O 90%; D2O 10%
sample_2: MlcB, [U-100% 13C; U-100% 15N], 1 mM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.5 mM; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
VNMRJ, Varian - collection
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts