BMRB Entry 18887
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18887
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Title: Solution structure of hypothetical protein lmo0427
Deposition date: 2012-12-10 Original release date: 2012-12-21
Authors: Zhang, Yongbo; Winsor, James; Radhakrishnan, Ishwar; Anderson, Wayne
Citation: Zhang, Yongbo; Winsor, James; Radhakrishnan, Ishwar; Anderson, Wayne. "Solution structure of hypothetical protein lmo0427" Not known ., .-..
Assembly members:
entity, polymer, 106 residues, 11395.479 Da.
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MKRKIIAVTACATGVAHTYM
AAQALKKGAKKMGNLIKVET
QGATGIENELTEKDVNIGEV
VIFAVDTKVRNKERFDGKVV
LEVPVSAPIKDAEKVINAAL
ALIDEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 462 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 766 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hypothetical protein lmo0427 | 1 |
Entities:
Entity 1, hypothetical protein lmo0427 106 residues - 11395.479 Da.
| 1 | MET | LYS | ARG | LYS | ILE | ILE | ALA | VAL | THR | ALA | ||||
| 2 | CYS | ALA | THR | GLY | VAL | ALA | HIS | THR | TYR | MET | ||||
| 3 | ALA | ALA | GLN | ALA | LEU | LYS | LYS | GLY | ALA | LYS | ||||
| 4 | LYS | MET | GLY | ASN | LEU | ILE | LYS | VAL | GLU | THR | ||||
| 5 | GLN | GLY | ALA | THR | GLY | ILE | GLU | ASN | GLU | LEU | ||||
| 6 | THR | GLU | LYS | ASP | VAL | ASN | ILE | GLY | GLU | VAL | ||||
| 7 | VAL | ILE | PHE | ALA | VAL | ASP | THR | LYS | VAL | ARG | ||||
| 8 | ASN | LYS | GLU | ARG | PHE | ASP | GLY | LYS | VAL | VAL | ||||
| 9 | LEU | GLU | VAL | PRO | VAL | SER | ALA | PRO | ILE | LYS | ||||
| 10 | ASP | ALA | GLU | LYS | VAL | ILE | ASN | ALA | ALA | LEU | ||||
| 11 | ALA | LEU | ILE | ASP | GLU | LYS |
Samples:
sample_1: Listeria monocytogenes EGD-e, [U-95% 13C; U-95% 15N], 1.2 mM; potassium phosphate 20 mM; sodium chloride 100 mM
sample_2: Listeria monocytogenes EGD-e, [U-95% 13C; U-95% 15N], 1.2 mM; potassium phosphate 20 mM; sodium chloride 100 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 6.7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
ARIA v1.2, Linge, O, . - refinement, structure solution
NMRPipe, Delaglio, Zhengrong and Bax - processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAC98506 CAK19805 CAR83139 CAS04216 CBY02952 |
| GB | AAT03227 ACK40550 ADB67216 ADB70305 AEH91456 |
| REF | NP_463956 WP_003722977 WP_003760426 WP_036088242 WP_047584151 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts