BMRB Entry 18892

Name: Solution structure of the helix II template boundary element from Tetrahymena telomerase RNA
Published: 2013-01-24

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PDB ID: 2m22
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18892
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the helix II template boundary element from Tetrahymena telomerase RNA PubMed: 16452301

Deposition date: 2012-12-11 Original release date: 2013-01-24

Authors: Cash, Darian; Richards, Rebecca; Theimer, Carla; Finger, David; Feigon, Juli

Citation: Richards, Rebecca; Theimer, Carla; Finger, David; Feigon, Juli. "Solution structure of the helix II template boundary element from Tetrahymena telomerase RNA" Nucleic Acids Res. 34, 816-825 (2006).

Assembly members:
helixII_telo_RNA, polymer, 23 residues, 7387.508 Da.

Natural source: Common Name: tetrahymena thermophila Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: tetrahymena thermophila

Experimental source: Production method: enzymatic synthesis Host organism: n/a

Entity Sequences (FASTA):
helixII_telo_RNA: GGCAGAUCUGUAAUAGAACU GCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	151
15N chemical shifts	34
1H chemical shifts	184

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	helixII_telo_RNA	1

Entities:

Entity 1, helixII_telo_RNA 23 residues - 7387.508 Da.

1

G

C

A

G

A

U

C

U

G

2

U

A

U

A

G

A

C

U

3

G

C

Samples:

sample_1: helixII_telo_RNA, unlabeled, 0.6 – 1.0 mM; sodium phosphate 10 mM; potassium chloride 30 mM; EDTA 50 uM; sodium azide 0.2%; H2O 90%; D2O 10%

sample_2: helixII_telo_RNA, 13C,15N uniformly labelled, 0.6 – 1.0 mM; sodium phosphate 10 mM; potassium chloride 30 mM; EDTA 50 uM; sodium azide 0.2%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 30 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 30 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
11echo and watergate	sample_1	isotropic	sample_conditions_1
2D TOCSY	sample_1	isotropic	sample_conditions_2
2D NOESY	sample_1	isotropic	sample_conditions_2
2D 15N-HMQC, 2D 15N-CPMG-NOESY, 2D JNN-HNN-COSY	sample_2	isotropic	sample_conditions_1
2D 13C-HSQCs	sample_2	isotropic	sample_conditions_2
2D HCCH COSY, 3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_2
3D 1H,13C HMQCs	sample_2	isotropic	sample_conditions_2

Software:

X-PLOR NIH v2.9.8, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

SPARKY, Goddard - data analysis, peak picking

xwinnmr, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 800 MHz
Bruker DRX 600 MHz
Bruker DRX 500 MHz