BMRB Entry 18909
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18909
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Title: Solution NMR structure of the SANT domain of human DNAJC2; Notrheast structural genomics consortium target HR8254a
Deposition date: 2012-12-18 Original release date: 2013-02-08
Authors: Lemak, Alexander; Yee, Adelinda; Houliston, Scott; Garcia, Maite; Ong, Michelle; Arrowsmith, Cheryl
Citation: Lemak, Alexander; Yee, Adelinda; Houliston, Scott; Garcia, Maite; Ong, Michelle; Arrowsmith, Cheryl. "NMR solution structure of the SANT domain of human DnaJC2." Not known ., .-..
Assembly members:
entity, polymer, 73 residues, 8308.837 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: QGFTPWTTEEQKLLEQALKT
YPVNTPERWKKIAEAVPGRT
KKDCMKRYKELVEMVKAKKA
AQEQVLNASRAKK
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 332 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 541 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hs00507 | 1 |
Entities:
Entity 1, hs00507 73 residues - 8308.837 Da.
| 1 | GLN | GLY | PHE | THR | PRO | TRP | THR | THR | GLU | GLU | ||||
| 2 | GLN | LYS | LEU | LEU | GLU | GLN | ALA | LEU | LYS | THR | ||||
| 3 | TYR | PRO | VAL | ASN | THR | PRO | GLU | ARG | TRP | LYS | ||||
| 4 | LYS | ILE | ALA | GLU | ALA | VAL | PRO | GLY | ARG | THR | ||||
| 5 | LYS | LYS | ASP | CYS | MET | LYS | ARG | TYR | LYS | GLU | ||||
| 6 | LEU | VAL | GLU | MET | VAL | LYS | ALA | LYS | LYS | ALA | ||||
| 7 | ALA | GLN | GLU | GLN | VAL | LEU | ASN | ALA | SER | ARG | ||||
| 8 | ALA | LYS | LYS |
Samples:
sample_1: hs00507, [U-13C; U-15N], 0.5 mM; TRIS 10 mM; sodium chloride 500 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; benzamidine 10 mM
sample_conditions_1: ionic strength: 500 mM; pH: 7.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - peak picking
FMC, Lemak,Steren,Llinas, Arrowsmith - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - data analysis
PSVS, Bhattacharya and Montelione - validation
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE00600 BAK62178 |
| EMBL | CAA66913 |
| GB | AAH00859 AAI14888 AAI25058 AAI39752 AAI60045 |
| REF | NP_001068805 NP_001123359 NP_001247727 NP_001267346 NP_001270315 |
| SP | Q1RMH9 Q4R8H2 Q99543 |
| TPG | DAA30686 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts