BMRB Entry 18952
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18952
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Serine 16 phosphorylated phospholamban pentamer, Hybrid solution and solid-state NMR structural ensemble PubMed: 24207128
Deposition date: 2013-01-15 Original release date: 2013-10-24
Authors: Vostrikov, Vitaly; Verardi, Raffaello; Veglia, Gianluigi
Citation: Vostrikov, Vitaly; Mote, Kaustubh; Verardi, Raffaello; Veglia, Gianluigi. "Structural Dynamics and Topology of Phosphorylated Phospholamban Homopentamer Reveal Its Role in the Regulation of Calcium Transport" Structure 21, 2119-2130 (2013).
Assembly members:
entity, polymer, 52 residues, 6179.515 Da.
PHOSPHOSERINE, non-polymer, 185.072 Da.
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MEKVQYLTRSAIRRAXTIEM
PQQARQNLQNLFINFCLILI
CLLLICIIVMLL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 49 |
| 1H chemical shifts | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Phospholamban_1 | 1 |
| 2 | Phospholamban_2 | 1 |
| 3 | Phospholamban_3 | 1 |
| 4 | Phospholamban_4 | 1 |
| 5 | Phospholamban_5 | 1 |
| 6 | PHOSPHOSERINE | 2 |
Entities:
Entity 1, Phospholamban_1 52 residues - 6179.515 Da.
| 1 | MET | GLU | LYS | VAL | GLN | TYR | LEU | THR | ARG | SER | ||||
| 2 | ALA | ILE | ARG | ARG | ALA | SEP | THR | ILE | GLU | MET | ||||
| 3 | PRO | GLN | GLN | ALA | ARG | GLN | ASN | LEU | GLN | ASN | ||||
| 4 | LEU | PHE | ILE | ASN | PHE | CYS | LEU | ILE | LEU | ILE | ||||
| 5 | CYS | LEU | LEU | LEU | ILE | CYS | ILE | ILE | VAL | MET | ||||
| 6 | LEU | LEU |
Entity 2, PHOSPHOSERINE - C3 H8 N O6 P - 185.072 Da.
| 1 | SEP |
Samples:
sample_1: Phospholamban, [U-100% 13C; U-100% 15N], 0.1 mM; DPC, [U-2H], 300 mM; sodium phosphate 20 mM; sodium chloride 120 mM; beta-mercaptoethanol 10 mM; sodium azide 0.02%; H2O 95%; D2O 5%
sample_2: Phospholamban, [U-13C; U-15N; U-2H], 0.15 mM; DPC 300 mM; sodium phosphate 20 mM; sodium chloride 120 mM; beta-mercaptoethanol 10 mM; sodium azide 0.02%; H2O 95%; D2O 5%
sample_3: Phospholamban, [U-100% 15N], 4 mg; DOPC 38 mg; DOPE 9 mg; D2O 100%
sample_4: Phospholamban, [U-100% 13C; U-100% 15N], 1 mg; DOPC 12 mg; DOPE 3 mg; MOPS 20 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O 100%
sample_conditions_1: pH: 6; temperature: 273 K
sample_conditions_2: pH: 7; temperature: 273 K
sample_conditions_3: pH: 7; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D PISEMA | sample_3 | anisotropic | sample_conditions_3 |
| 2D DARR | sample_4 | solid | sample_conditions_2 |
Software:
NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.114, Goddard - data analysis
MolProbity, David C. Richardson - data analysis
PSVS, Bhattacharya and Montelione - data analysis
X-PLOR NIH v2.33, Schwieters, Kuszewski, Tjandra and Clore - structure solution
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 700 MHz
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22237 BAC30680 BAC34880 BAE25168 |
| EMBL | CAA50394 CAA68730 |
| GB | AAA31445 AAA41849 AAB21903 AAB23706 AAH61097 |
| REF | NP_001076090 NP_001135399 NP_001177823 NP_073198 NP_075618 |
| SP | P61014 P61015 P61016 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts