BMRB Entry 18967
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18967
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Title: Solution structure of the C-terminal zinc-binding domain of HPV51 oncoprotein E6 PubMed: 23638119
Deposition date: 2013-01-21 Original release date: 2013-05-13
Authors: Mischo, Andre; Ohlenschlager, Oliver; Gorlach, Matthias
Citation: Mischo, Andre; Ohlenschlager, Oliver; Hortschansky, Peter; Ramachandran, Ramadurai; Gorlach, Matthias. "Structural insights into a wildtype domain of the oncoprotein E6 and its interaction with a PDZ domain" PLOS One 8, e62584-e62584 (2013).
Assembly members:
E6, polymer, 76 residues, 8951.278 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human papillomavirus HPV Taxonomy ID: 10566 Superkingdom: Viruses Kingdom: not available Genus/species: Human papillomavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E6: GSHMSRSVYGTTLEAITKKS
LYDLSIRCHRCQRPLGPEEK
QKLVDEKKRFHEIAGRWTGQ
CANCWQRTRQRNETQV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 337 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 527 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E6 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, E6 76 residues - 8951.278 Da.
Residues 1-4 represent a non-native sequence originating from protease cleavage
| 1 | GLY | SER | HIS | MET | SER | ARG | SER | VAL | TYR | GLY | ||||
| 2 | THR | THR | LEU | GLU | ALA | ILE | THR | LYS | LYS | SER | ||||
| 3 | LEU | TYR | ASP | LEU | SER | ILE | ARG | CYS | HIS | ARG | ||||
| 4 | CYS | GLN | ARG | PRO | LEU | GLY | PRO | GLU | GLU | LYS | ||||
| 5 | GLN | LYS | LEU | VAL | ASP | GLU | LYS | LYS | ARG | PHE | ||||
| 6 | HIS | GLU | ILE | ALA | GLY | ARG | TRP | THR | GLY | GLN | ||||
| 7 | CYS | ALA | ASN | CYS | TRP | GLN | ARG | THR | ARG | GLN | ||||
| 8 | ARG | ASN | GLU | THR | GLN | VAL |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: E6, [U-100% 13C; U-100% 15N], 1.13 mM; sodium chloride 90 mM; L-arginine 45 mM; L-glutamate 45 mM; DTT 9 mM; sodium azide 0.05 w/v; H2O 90%; D2O 10%
sample_2: E6, [U-100% 13C; U-100% 15N], 0.9 mM; sodium chloride 90 mM; L-arginine 45 mM; L-glutamate 45 mM; DTT 9 mM; sodium azide 0.05 w/v; D2O 100%
sample_conditions_1: ionic strength: 180 mM; pH: 7.4; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CARA, Keller and Wuthrich - data analysis
TOPSPIN, Bruker Biospin - collection & processing
NMR spectrometers:
- Bruker AvanceIII 750 MHz
- Bruker AvanceIII 600 MHz
Related Database Links:
| UniProtKB | P26554 |
| PDB | |
| EMBL | CRH69907 |
| GB | ALJ32861 ALJ32868 ALJ32875 ALJ32882 ALJ32889 |
| SP | P26554 |
Download simulated HSQC data in one of the following formats:
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