BMRB Entry 18986
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18986
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Title: 1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricus PubMed: 23934138
Deposition date: 2013-01-29 Original release date: 2013-09-30
Authors: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias
Citation: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias. "(1)H, (15)N and (13)C chemical shift assignments for the winged helix domains of two archeal MCM C-termini." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
C-terminus_of_the_minichromosome_maintenance_protein_MCM, polymer, 87 residues, 9674.474 Da.
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
C-terminus_of_the_minichromosome_maintenance_protein_MCM: GSHMGESGKIDIDTIMTGKP
KSAREKMMKIIEIIDSLAVS
SECAKVKDILKEAQQVGIEK
SNIEKLLTDMRKSGIIYEAK
PECYKKV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 391 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 637 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C-terminus of the minichromosome maintenance protein MCM | 1 |
Entities:
Entity 1, C-terminus of the minichromosome maintenance protein MCM 87 residues - 9674.474 Da.
| 1 | GLY | SER | HIS | MET | GLY | GLU | SER | GLY | LYS | ILE | ||||
| 2 | ASP | ILE | ASP | THR | ILE | MET | THR | GLY | LYS | PRO | ||||
| 3 | LYS | SER | ALA | ARG | GLU | LYS | MET | MET | LYS | ILE | ||||
| 4 | ILE | GLU | ILE | ILE | ASP | SER | LEU | ALA | VAL | SER | ||||
| 5 | SER | GLU | CYS | ALA | LYS | VAL | LYS | ASP | ILE | LEU | ||||
| 6 | LYS | GLU | ALA | GLN | GLN | VAL | GLY | ILE | GLU | LYS | ||||
| 7 | SER | ASN | ILE | GLU | LYS | LEU | LEU | THR | ASP | MET | ||||
| 8 | ARG | LYS | SER | GLY | ILE | ILE | TYR | GLU | ALA | LYS | ||||
| 9 | PRO | GLU | CYS | TYR | LYS | LYS | VAL |
Samples:
sample_1: C-terminus of the minichromosome maintenance protein MCM, [U-100% 13C; U-100% 15N], 700 uM; potassium phosphate 10 mM; sodium chloride 150 mM; sodium azide 0.05 w/v
sample_2: C-terminus of the minichromosome maintenance protein MCM, [U-100% 13C; U-100% 15N], 700 uM; potassium phosphate 10 mM; sodium chloride 150 mM; sodium azide 0.05 w/v
sample_conditions_1: ionic strength: 160 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
Software:
CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution
TOPSPIN, Bruker Biospin - collection & processing
CcpNMR, CCPN - data analysis
OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement
NMR spectrometers:
- Bruker AvanceIII 600 MHz
Related Database Links:
| UniProtKB | Q9UXG1 |
| PDB | |
| EMBL | CAB57529 |
| GB | AAK41071 ACP35470 ACP38121 ACP45628 ACP48570 |
| REF | WP_009991364 WP_012711366 WP_012713703 WP_012716161 WP_012717457 |
| SP | Q9UXG1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts