BMRB Entry 19077
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19077
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Title: 1H, 13C and 15N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domain of the human deubiquitinase USP28 PubMed: 23754699
Deposition date: 2013-03-06 Original release date: 2014-02-13
Authors: Wen, Yi; Cui, Rong; Zhang, Huaqun; Zhang, Naixia
Citation: Wen, Yi; Cui, Rong; Zhang, Huaqun; Zhang, Naixia. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domains of the human deubiquitinase Usp28." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
USP28, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
USP28: GSMTAELQQDDAAGAADGHG
SSCQMLLNQLREITGIQDPS
FLHEALKASNGDITQAVSLL
TDERVKEPSQDTVATEPSEV
EGSAANKEVLAKVIDLTHDN
KDDLQAAIALSLLESPKIQA
DGVDKLAAALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 487 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 764 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | USP28 monomer | 1 |
Entities:
Entity 1, USP28 monomer 137 residues - Formula weight is not available
The first two residues GS and the last fifteen residues VDKLAAALEHHHHHH are cloning artifacts.
| 1 | GLY | SER | MET | THR | ALA | GLU | LEU | GLN | GLN | ASP | ||||
| 2 | ASP | ALA | ALA | GLY | ALA | ALA | ASP | GLY | HIS | GLY | ||||
| 3 | SER | SER | CYS | GLN | MET | LEU | LEU | ASN | GLN | LEU | ||||
| 4 | ARG | GLU | ILE | THR | GLY | ILE | GLN | ASP | PRO | SER | ||||
| 5 | PHE | LEU | HIS | GLU | ALA | LEU | LYS | ALA | SER | ASN | ||||
| 6 | GLY | ASP | ILE | THR | GLN | ALA | VAL | SER | LEU | LEU | ||||
| 7 | THR | ASP | GLU | ARG | VAL | LYS | GLU | PRO | SER | GLN | ||||
| 8 | ASP | THR | VAL | ALA | THR | GLU | PRO | SER | GLU | VAL | ||||
| 9 | GLU | GLY | SER | ALA | ALA | ASN | LYS | GLU | VAL | LEU | ||||
| 10 | ALA | LYS | VAL | ILE | ASP | LEU | THR | HIS | ASP | ASN | ||||
| 11 | LYS | ASP | ASP | LEU | GLN | ALA | ALA | ILE | ALA | LEU | ||||
| 12 | SER | LEU | LEU | GLU | SER | PRO | LYS | ILE | GLN | ALA | ||||
| 13 | ASP | GLY | VAL | ASP | LYS | LEU | ALA | ALA | ALA | LEU | ||||
| 14 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: D20 10%; H20 90%; USP-28, [U-15N], 0.8 – 1.0 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; NaNH3 0.02%
sample_2: D20 10%; H20 90%; USP-28, [U-15N; U-13C], 0.8 – 1.0 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; NaNH3 0.02%
sample_3: D20 100%; USP-28, [U-15N; U-13C], 0.8 – 1.0 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; NaNH3 0.02%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_3 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_3 | isotropic | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 18560 |
| PDB | |
| DBJ | BAG65214 |
| GB | AAH65928 AAK58565 ACA06098 ACA06099 AIC60511 |
| REF | NP_065937 XP_001085291 XP_002799842 XP_003253212 XP_004052203 |
| SP | Q96RU2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts