BMRB Entry 19084
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19084
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Title: The Mengovirus Leader protein
Deposition date: 2013-03-12 Original release date: 2013-04-17
Authors: Cornilescu, Claudia; Porter, Frederick; Zao, Kate Qin; Davis, Valarie; Palmenberg, Ann; Markley, John
Citation: Bacot-Davis, Valjean; Ciomperlik, J.; Basta, H.; Cornilescu, Claudia; Palmenberg, Ann. "Solution Structures of Mengovirus Leader Protein, its Phosphorylated Derivatives, and in Complex with Nuclear Transport Protein, RanGTPase" Proc. Nat. Acad. Sci, U.S.A. ., .-. (2014).
Assembly members:
full_length_mengoL, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: Mengo virus Taxonomy ID: 12104 Superkingdom: Viruses Kingdom: not available Genus/species: Cardiovirus Encephalomyocarditis virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
full_length_mengoL: GSTAMATTMEQEICAHSMTF
EECPKCSALQYRNGFYLLKY
DEEWYPEELLTDGEDDVFDP
DLDMEVVFETQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 251 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 231 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mengovirus | 1 |
Entities:
Entity 1, mengovirus 71 residues - Formula weight is not available
| 1 | GLY | SER | THR | ALA | MET | ALA | THR | THR | MET | GLU | ||||
| 2 | GLN | GLU | ILE | CYS | ALA | HIS | SER | MET | THR | PHE | ||||
| 3 | GLU | GLU | CYS | PRO | LYS | CYS | SER | ALA | LEU | GLN | ||||
| 4 | TYR | ARG | ASN | GLY | PHE | TYR | LEU | LEU | LYS | TYR | ||||
| 5 | ASP | GLU | GLU | TRP | TYR | PRO | GLU | GLU | LEU | LEU | ||||
| 6 | THR | ASP | GLY | GLU | ASP | ASP | VAL | PHE | ASP | PRO | ||||
| 7 | ASP | LEU | ASP | MET | GLU | VAL | VAL | PHE | GLU | THR | ||||
| 8 | GLN |
Samples:
sample_1: full length mengoL, [U-13C; U-15N], 0.7 – 1 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D-(HB)CB(CGCDCE)HE | sample_1 | isotropic | sample_conditions_1 |
| 2D-(HB)CB(CGCD)HD | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 900 MHz
Related Database Links:
| BMRB | 19855 19857 19858 |
| PDB | |
| EMBL | CAE84564 CAE84565 CAE84566 CAE84567 |
| GB | AAA46547 AAB59755 ABB97066 ABI15777 |
| SP | P12296 P32540 |
Download simulated HSQC data in one of the following formats:
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