BMRB Entry 19094
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19094
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Title: Enterocin 7A PubMed: 23725536
Deposition date: 2013-03-16 Original release date: 2013-04-16
Authors: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John
Citation: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John. "Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide" Biochemistry 52, 3987-3994 (2013).
Assembly members:
Enterocin_7A, polymer, 44 residues, 5213.414 Da.
Natural source: Common Name: Firmicutes Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: purified from the natural source Host organism: Enterococcus faecalis
Entity Sequences (FASTA):
Enterocin_7A: XGAIAKLVAKFGWPIVKKYY
KQIMQFIGEGWAINKIIDWI
KKHI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 316 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Enterocin 7A | 1 |
Entities:
Entity 1, Enterocin 7A 44 residues - 5213.414 Da.
| 1 | FME | GLY | ALA | ILE | ALA | LYS | LEU | VAL | ALA | LYS | ||||
| 2 | PHE | GLY | TRP | PRO | ILE | VAL | LYS | LYS | TYR | TYR | ||||
| 3 | LYS | GLN | ILE | MET | GLN | PHE | ILE | GLY | GLU | GLY | ||||
| 4 | TRP | ALA | ILE | ASN | LYS | ILE | ILE | ASP | TRP | ILE | ||||
| 5 | LYS | LYS | HIS | ILE |
Samples:
sample_1: Enterocin 7A 0.8 mM; DSS 0.01 w/v; H2O 90%; D2O 10%
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian VNMRS 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAF74788 |
| EMBL | CAA11520 |
| GB | ABB46250 ABC95646 ABL11217 ACM68960 AFU07735 |
| REF | WP_002293184 YP_001966103 YP_009082434 |