BMRB Entry 19095
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19095
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Title: Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single-stranded DNA binding protein from Sulfolobus Solfataricus PubMed: 23749431
Deposition date: 2013-03-17 Original release date: 2013-04-17
Authors: Gamsjaeger, Roland; Kariawasam, Ruvini; Touma, Christine; White, Malcolm; Cubeddu, Liza
Citation: Gamsjaeger, Roland; Kariawasam, Ruvini; Touma, Christine; Kwan, Ann; White, Malcolm; Cubeddu, Liza. "Backbone and side-chain (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
SsoSSB, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Crenarchaeotes Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus Solfataricus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SsoSSB: MEEKVGNLKPNMESVNVTVR
VLEASEARQIQTKNGVRTIS
EAIVGDETGRVKLTLWGKHA
GSIKEGQVVKIENAWTTAFK
GQVQLNAGSKTKIAEASEDG
FPESSQIPENTPTARRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 505 |
| 15N chemical shifts | 133 |
| 1H chemical shifts | 829 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SsoSSB | 1 |
Entities:
Entity 1, SsoSSB 117 residues - Formula weight is not available
| 1 | MET | GLU | GLU | LYS | VAL | GLY | ASN | LEU | LYS | PRO | ||||
| 2 | ASN | MET | GLU | SER | VAL | ASN | VAL | THR | VAL | ARG | ||||
| 3 | VAL | LEU | GLU | ALA | SER | GLU | ALA | ARG | GLN | ILE | ||||
| 4 | GLN | THR | LYS | ASN | GLY | VAL | ARG | THR | ILE | SER | ||||
| 5 | GLU | ALA | ILE | VAL | GLY | ASP | GLU | THR | GLY | ARG | ||||
| 6 | VAL | LYS | LEU | THR | LEU | TRP | GLY | LYS | HIS | ALA | ||||
| 7 | GLY | SER | ILE | LYS | GLU | GLY | GLN | VAL | VAL | LYS | ||||
| 8 | ILE | GLU | ASN | ALA | TRP | THR | THR | ALA | PHE | LYS | ||||
| 9 | GLY | GLN | VAL | GLN | LEU | ASN | ALA | GLY | SER | LYS | ||||
| 10 | THR | LYS | ILE | ALA | GLU | ALA | SER | GLU | ASP | GLY | ||||
| 11 | PHE | PRO | GLU | SER | SER | GLN | ILE | PRO | GLU | ASN | ||||
| 12 | THR | PRO | THR | ALA | ARG | ARG | ARG |
Samples:
sample_1: SsoSSB, [U-100% 13C; U-100% 15N], 0.8 – 1.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 60 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| GB | AAK42515 ACP34347 ACP37052 ACP44458 ACP54191 |
| REF | WP_009989484 WP_012710339 |
| SP | Q97W73 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts