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Biological Magnetic Resonance Data Bank


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BMRB Entry 19111

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19111
MolProbity Validation Chart

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Title: USP25   PubMed: 23754700

Deposition date: 2013-03-27 Original release date: 2013-08-16

Authors: Shi, Li; Zhang, Naixia; Wen, Yi

Citation: Shi, Li; Wen, Yi; Zhang, Naixia. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domains of USP25."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
USP25, polymer, 166 residues, Formula weight is not available

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
USP25: MGSSHHHHHHSSGLVPRGSH MTVEQNVLQQSAAQKHQQTF LNQLREITGINDTQILQQAL KDSNGNLELAVAFLTAKNAK TPQQEETTYYQTALPGNDRY ISVGSQADTNVIDLTGDDKD DLQRAIALSLAESNRAFRET GITDEEQAISRVLEASIAEN KACLKR

Data sets:
Data typeCount
13C chemical shifts603
15N chemical shifts162
1H chemical shifts967

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1USP251

Entities:

Entity 1, USP25 166 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METTHRVALGLUGLNASNVALLEUGLNGLN
4   SERALAALAGLNLYSHISGLNGLNTHRPHE
5   LEUASNGLNLEUARGGLUILETHRGLYILE
6   ASNASPTHRGLNILELEUGLNGLNALALEU
7   LYSASPSERASNGLYASNLEUGLULEUALA
8   VALALAPHELEUTHRALALYSASNALALYS
9   THRPROGLNGLNGLUGLUTHRTHRTYRTYR
10   GLNTHRALALEUPROGLYASNASPARGTYR
11   ILESERVALGLYSERGLNALAASPTHRASN
12   VALILEASPLEUTHRGLYASPASPLYSASP
13   ASPLEUGLNARGALAILEALALEUSERLEU
14   ALAGLUSERASNARGALAPHEARGGLUTHR
15   GLYILETHRASPGLUGLUGLNALAILESER
16   ARGVALLEUGLUALASERILEALAGLUASN
17   LYSALACYSLEULYSARG

Samples:

sample_1: USP25, [U-99% 13C; U-99% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_2: USP25, [U-99% 15N], 0.8 mM; H2O 90%; D2O 10%

sample_3: USP25, [U-99% 13C], 0.8 mM; D2O 100%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HCCH-COSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAB27849 BAC29438
GB AAF24998 AAF32263 AAF32264 AAH15930 AAH48171
REF NP_001269970 NP_001269971 NP_037528 NP_038946 XP_001155737
SP P57080 Q9UHP3
TPG DAA33643

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts