BMRB Entry 19170
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19170
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Title: solution structure of the calmodulin-binding domain of plant calcium-ATPase ACA8
Deposition date: 2013-04-16 Original release date: 2014-04-14
Authors: Jamshidiha, Mostafa; Ishida, Hiroaki; Gifford, Jessica; Vogel, Hans
Citation: Jamshidiha, Mostafa; Ishida, Hiroaki; Gifford, Jessica; Vogel, Hans. "Structural characterization of the interaction between a plant calmodulin and three distinct calcium-ATPase pumps" Not known ., .-..
Assembly members:
ACA8, polymer, 25 residues, 3110.716 Da.
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: chemical synthesis Host organism: Arabidopsis thaliana
Entity Sequences (FASTA):
ACA8: RLQQWRKAALVLNASRRFRY
TLDLK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 206 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ACA8 | 1 |
Entities:
Entity 1, ACA8 25 residues - 3110.716 Da.
| 1 | ARG | LEU | GLN | GLN | TRP | ARG | LYS | ALA | ALA | LEU | ||||
| 2 | VAL | LEU | ASN | ALA | SER | ARG | ARG | PHE | ARG | TYR | ||||
| 3 | THR | LEU | ASP | LEU | LYS |
Samples:
sample_1: D2O, [U-100% 2H], 10%; DSS, [U-100% 2H], 5 mM; H2O 60%; TFE, [U-100% 2H], 30%; DTT, [U-100% 2H], 10 mM; trifluoroethanol 40%; ACA8 mM
sample_conditions_1: ionic strength: 0 M; pH: 4.2; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz