BMRB Entry 19179
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19179
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Title: ORF PP_3909 from Pseudomonas putida KT2440 encoding a protein similar to bacteriophage lambda ea8.5 PubMed: 23672713
Deposition date: 2013-04-18 Original release date: 2013-06-10
Authors: Donaldson, Logan
Citation: Kwan, Jamie; Smirnova, Ekaterina; Khazai, Shahin; Evanics, Ferenc; Maxwell, Karen; Donaldson, Logan. "The Solution Structures of Two Prophage Homologues of the Bacteriophage Ea8.5 Protein Reveal a Newly Discovered Hybrid Homeodomain/Zinc-Finger Fold." Biochemistry 52, 3612-3614 (2013).
Assembly members:
mol1, polymer, 92 residues, 10196.0386 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: g-proteobacteria Taxonomy ID: 303 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas putida
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
mol1: MGGIKRLMEEEDAKYSEAVY
IAIEAGTLAECEVHEGTYFS
DSGDISEAEELAREKFEKGE
VSNFDDVEELVKKVVAVCEE
LGAEECFSCDFD
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 307 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 477 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mol1 | 1 |
| 2 | Zinc ions | 2 |
Entities:
Entity 1, mol1 92 residues - 10196.0386 Da.
| 1 | MET | GLY | GLY | ILE | LYS | ARG | LEU | MET | GLU | GLU | ||||
| 2 | GLU | ASP | ALA | LYS | TYR | SER | GLU | ALA | VAL | TYR | ||||
| 3 | ILE | ALA | ILE | GLU | ALA | GLY | THR | LEU | ALA | GLU | ||||
| 4 | CYS | GLU | VAL | HIS | GLU | GLY | THR | TYR | PHE | SER | ||||
| 5 | ASP | SER | GLY | ASP | ILE | SER | GLU | ALA | GLU | GLU | ||||
| 6 | LEU | ALA | ARG | GLU | LYS | PHE | GLU | LYS | GLY | GLU | ||||
| 7 | VAL | SER | ASN | PHE | ASP | ASP | VAL | GLU | GLU | LEU | ||||
| 8 | VAL | LYS | LYS | VAL | VAL | ALA | VAL | CYS | GLU | GLU | ||||
| 9 | LEU | GLY | ALA | GLU | GLU | CYS | PHE | SER | CYS | ASP | ||||
| 10 | PHE | ASP |
Entity 2, Zinc ions - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: mol1, [U-99% 13C; U-99% 15N], 0.6 mM; H20 90%; D20 10%
condition_1: ionic strength: 0.150 M; pH: 7.800; pressure: 1.000 atm; temperature: 293.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | sample_1 | isotropic | condition_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | condition_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | condition_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | condition_1 |
| 3D C(CO)NH | sample_1 | isotropic | condition_1 |
| 3D HNCO | sample_1 | isotropic | condition_1 |
| 3D HNCACB | sample_1 | isotropic | condition_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | condition_1 |
| 3D HN(CA)CO | sample_1 | isotropic | condition_1 |
| 3D H(CCO)NH | sample_1 | isotropic | condition_1 |
| 2D CBHD | sample_1 | isotropic | condition_1 |
| 2D CBHE | sample_1 | isotropic | condition_1 |
| 3D (H)C(CO)NH | sample_1 | isotropic | condition_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | condition_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | condition_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | condition_1 |
Software:
ANALYSIS v2.2, CCPN - data analysis
CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian NMRS 600 MHz
Related Database Links:
| GenBank | 24987239 |
| PDB | |
| GB | AAN69503 KMU94681 KMY31145 |
| REF | NP_746039 WP_010954735 |
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