BMRB Entry 19183
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19183
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Title: Trp-cage 16b P12W: a Hyperstable Miniprotein PubMed: 24376912
Deposition date: 2013-04-19 Original release date: 2013-12-23
Authors: Williams, Dawn; Andersen, Niels; Kier, Brandon
Citation: Byrne, Aimee; Kier, Brandon; Williams, D.; Scian, Michele; Andersen, Niels. "Circular Permutation of the Trp-cage: Fold Rescue upon Addition of a Hydrophobic Staple." RSC Adv. 2013, .-. (2013).
Assembly members:
entity_1, polymer, 20 residues, 2131.306 Da.
D-ALANINE, non-polymer, 89.093 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
entity_1: DAYAQWLADXGWASXRPPPS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Trp-cage 16b P12W: a Hyperstable Miniprotein | 1 |
Entities:
Entity 1, Trp-cage 16b P12W: a Hyperstable Miniprotein 20 residues - 2131.306 Da.
| 1 | ASP | ALA | TYR | ALA | GLN | TRP | LEU | ALA | ASP | DAL | |
| 2 | GLY | TRP | ALA | SER | DAL | ARG | PRO | PRO | PRO | SER |
Samples:
sample_1: Trp-cage 16b P12W: a Hyperstable Miniprotein 1.2 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.11 M; pH: 7; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker DMX 800 MHz
Related Database Links:
| PDB |