BMRB Entry 19185
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19185
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Title: Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens
Deposition date: 2013-04-22 Original release date: 2013-10-17
Authors: Reardon, Patrick; Mueller, Karl
Citation: Reardon, Patrick; Mueller, Karl. "Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens" Not known ., .-..
Assembly members:
PilA, polymer, 61 residues, 6576.573 Da.
Natural source: Common Name: d-proteobacteria Taxonomy ID: 35554 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacter sulfurreducens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PilA: FTLIELLIVVAIIGILAAIA
IPQFSAYRVKAYNSAASSDL
RNLKTALESAFADDQTYPPE
S
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 221 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 396 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PilA | 1 |
Entities:
Entity 1, PilA 61 residues - 6576.573 Da.
| 1 | PHE | THR | LEU | ILE | GLU | LEU | LEU | ILE | VAL | VAL | ||||
| 2 | ALA | ILE | ILE | GLY | ILE | LEU | ALA | ALA | ILE | ALA | ||||
| 3 | ILE | PRO | GLN | PHE | SER | ALA | TYR | ARG | VAL | LYS | ||||
| 4 | ALA | TYR | ASN | SER | ALA | ALA | SER | SER | ASP | LEU | ||||
| 5 | ARG | ASN | LEU | LYS | THR | ALA | LEU | GLU | SER | ALA | ||||
| 6 | PHE | ALA | ASP | ASP | GLN | THR | TYR | PRO | PRO | GLU | ||||
| 7 | SER |
Samples:
sample_1: PilA, [U-13C; U-15N], 1 mM; DHPC, [U-2H], 200 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ v3.2, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
CARA, Keller and Wuthrich - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Varian VNMRs 800 MHz
- Varian VNMRs 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts