BMRB Entry 19187
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19187
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Title: 1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Methanothermobacter thermautotrophicus PubMed: 23934138
Deposition date: 2013-04-24 Original release date: 2013-09-30
Authors: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias
Citation: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias. "(1)H, (15)N and (13)C chemical shift assignments for the winged helix domains of two archeal MCM C-termini." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
C-terminus_MCM, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Methanothermobacter thermautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: not available Genus/species: Methanothermobacter thermautotrophicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
C-terminus_MCM: GAMGETGKIDIDKVEGRTPK
SERDKFRLLLELIKEYEDDY
GGRAPTNILITEMMDRYNVS
EEKVEELIRILKDKGAIFEP
ARGYLKIV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 405 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 645 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Methanothermobacter thermautotrophicus MCM C-terminus | 1 |
Entities:
Entity 1, Methanothermobacter thermautotrophicus MCM C-terminus 88 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | GLU | THR | GLY | LYS | ILE | ASP | ||||
| 2 | ILE | ASP | LYS | VAL | GLU | GLY | ARG | THR | PRO | LYS | ||||
| 3 | SER | GLU | ARG | ASP | LYS | PHE | ARG | LEU | LEU | LEU | ||||
| 4 | GLU | LEU | ILE | LYS | GLU | TYR | GLU | ASP | ASP | TYR | ||||
| 5 | GLY | GLY | ARG | ALA | PRO | THR | ASN | ILE | LEU | ILE | ||||
| 6 | THR | GLU | MET | MET | ASP | ARG | TYR | ASN | VAL | SER | ||||
| 7 | GLU | GLU | LYS | VAL | GLU | GLU | LEU | ILE | ARG | ILE | ||||
| 8 | LEU | LYS | ASP | LYS | GLY | ALA | ILE | PHE | GLU | PRO | ||||
| 9 | ALA | ARG | GLY | TYR | LEU | LYS | ILE | VAL |
Samples:
sample_1: C-terminus of the minichromosome maintenance protein MCM of Methanothermobacter thermautotrophicus, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; H20 90%; D2O 10%
sample_2: C-terminus of the minichromosome maintenance protein MCM of Methanothermobacter thermautotrophicus, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; D2O 100%
sample_conditions_1: ionic strength: 160 mM; pH: 6.2; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CcpNMR, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AvanceIII 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts