BMRB Entry 19237
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19237
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Title: Solution structure of AhPDF1 from Arabidopsis halleri
Deposition date: 2013-05-13 Original release date: 2014-05-12
Authors: Meindre, Fanny; Paquet, Francoise; Landon, Celine
Citation: Meindre, Fanny; Paquet, Francoise; Landon, Celine; Meindre, Fanny; Paquet, Francoise; Landon, Celine. "Folding and structural studies of the protein AhPDF1" Not known ., .-..
Assembly members:
AhPDF1, polymer, 51 residues, 5699.622 Da.
Natural source: Common Name: Eudicots Taxonomy ID: 81970 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Arabidopsis halleri
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
AhPDF1: XRLCEKPSGTWSGVCGNNGA
CRNQCIRLEKARHGSCNYVF
PAHKCICYFPC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 128 |
| 15N chemical shifts | 30 |
| 1H chemical shifts | 344 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AhPDF1 from Arabidopsis halleri | 1 |
Entities:
Entity 1, AhPDF1 from Arabidopsis halleri 51 residues - 5699.622 Da.
| 1 | PCA | ARG | LEU | CYS | GLU | LYS | PRO | SER | GLY | THR | ||||
| 2 | TRP | SER | GLY | VAL | CYS | GLY | ASN | ASN | GLY | ALA | ||||
| 3 | CYS | ARG | ASN | GLN | CYS | ILE | ARG | LEU | GLU | LYS | ||||
| 4 | ALA | ARG | HIS | GLY | SER | CYS | ASN | TYR | VAL | PHE | ||||
| 5 | PRO | ALA | HIS | LYS | CYS | ILE | CYS | TYR | PHE | PRO | ||||
| 6 | CYS |
Samples:
sample_1: AhPDF1 1.3 mM; H2O 90%; D2O 10%
sample_2: AhPDF1 1.3 mM; D2O 100%
sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 273 K
sample_conditions_2: pH: 4.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
CcpNMR v2.2.2, CCPN - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
ARIA v2.3, Linge, O, . - refinement, structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts