BMRB Entry 19274
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19274
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Title: Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC
Deposition date: 2013-05-28 Original release date: 2014-12-22
Authors: Liburd, Janine; Miller, Emily; Langelaan, David; Chitayat, Seth; Crawley, Scott; Cote, Graham; Smith, Steven
Citation: Liburd, Janine; Miller, Emily; Langelaan, David; Chitayat, Seth; Crawley, Scott; Cote, Graham; Smith, Steven. "Solution Structure of the Dictyostelium discodieum Myosin Light Chain, MlcC" Not known ., .-..
Assembly members:
entity, polymer, 77 residues, 8918.021 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GSHMNHINTKAQVIEAFKVF
DRDGNGYVTVDYLRKVLNEL
GDMMPADEIEEMIYEADPQN
SGYVQYETFVGMLFLWD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 320 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 509 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dictyostelium discodieum Myosin Light Chain, MlcC | 1 |
Entities:
Entity 1, Dictyostelium discodieum Myosin Light Chain, MlcC 77 residues - 8918.021 Da.
| 1 | GLY | SER | HIS | MET | ASN | HIS | ILE | ASN | THR | LYS | ||||
| 2 | ALA | GLN | VAL | ILE | GLU | ALA | PHE | LYS | VAL | PHE | ||||
| 3 | ASP | ARG | ASP | GLY | ASN | GLY | TYR | VAL | THR | VAL | ||||
| 4 | ASP | TYR | LEU | ARG | LYS | VAL | LEU | ASN | GLU | LEU | ||||
| 5 | GLY | ASP | MET | MET | PRO | ALA | ASP | GLU | ILE | GLU | ||||
| 6 | GLU | MET | ILE | TYR | GLU | ALA | ASP | PRO | GLN | ASN | ||||
| 7 | SER | GLY | TYR | VAL | GLN | TYR | GLU | THR | PHE | VAL | ||||
| 8 | GLY | MET | LEU | PHE | LEU | TRP | ASP |
Samples:
sample_1: entity 1 mM; HEPES 10 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 10%; H2O 90%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMRView, Johnson, One Moon Scientific - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts