BMRB Entry 19342
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19342
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Title: Transmembrane-cytosolic part of Trop2 explored by NMR and Molecular Dynamics
Deposition date: 2013-07-08 Original release date: 2014-07-07
Authors: Vidmar, Tilen; Pav i, Miha; Ilc, Gregor; Plavec, Janez; Lenar i, Brigita
Citation: Vidmar, Tilen; Pav i, Miha; Ilc, Gregor; Plavec, Janez; Lenar i, Brigita. "Transmembrane-cytosolic part of Trop2 explored by NMR and Molecular Dynamics" Not known ., .-..
Assembly members:
entity, polymer, 26 residues, 3096.678 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: TNRRKSGKYKKVEIKELGEL
RKEPSL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 79 |
| 15N chemical shifts | 25 |
| 1H chemical shifts | 193 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Transmembrane-cytosolic part of Trop2 | 1 |
Entities:
Entity 1, Transmembrane-cytosolic part of Trop2 26 residues - 3096.678 Da.
| 1 | THR | ASN | ARG | ARG | LYS | SER | GLY | LYS | TYR | LYS | ||||
| 2 | LYS | VAL | GLU | ILE | LYS | GLU | LEU | GLY | GLU | LEU | ||||
| 3 | ARG | LYS | GLU | PRO | SER | LEU |
Samples:
sample_1: entity mM; DSS 5.5 mM; TFE 70%; H2O 30%
sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
YASARA, Krieger E. - refinement
NMR spectrometers:
- Varian VNMRS 900 MHz
Related Database Links:
| BMRB | 25274 |
| PDB | |
| EMBL | CAA31781 CAA54799 CAA54801 CAG47056 |
| GB | AAA52505 AAH09409 AAP35919 AAP88905 AAX29730 |
| REF | NP_002344 XP_002810812 XP_003265191 XP_003824268 XP_003892008 |
| SP | P09758 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts