BMRB Entry 19525
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19525
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Title: Solution structure of the Nt. GR-RBP1 RRM domain
Deposition date: 2013-09-24 Original release date: 2013-09-30
Authors: Kahn, F.; Boelens, R.; Saqlan, S.; van Ingen, Hugo
Citation: Kahn, F.; Boelens, R.; Saqlan, S.; van Ingen, Hugo. "Structural basis of nucleic acid binding by Nicotiana tabacum glycine-rich RNA binding protein: implications for its RNA chaperone function" To be published ., .-..
Assembly members:
RNA-BINDING_GLYCINE-RICH_PROTEIN, polymer, 86 residues, 9522.4633 Da.
Natural source: Common Name: Tobacco Taxonomy ID: 4097 Superkingdom: Eukaryota Kingdom: not available Genus/species: Nicotiana Tabacum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RNA-BINDING_GLYCINE-RICH_PROTEIN: GMAEVEYRCFVGGLAWATTD
QTLGEAFSQFGEILDSKIIN
DRETGRSRGFGFVTFKDEKA
MRDAIEGMNGQDLDGRNITV
NEAQSR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 362 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 563 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA-BINDING GLYCINE-RICH PROTEIN | 1 |
Entities:
Entity 1, RNA-BINDING GLYCINE-RICH PROTEIN 86 residues - 9522.4633 Da.
| 1 | GLY | MET | ALA | GLU | VAL | GLU | TYR | ARG | CYS | PHE | ||||
| 2 | VAL | GLY | GLY | LEU | ALA | TRP | ALA | THR | THR | ASP | ||||
| 3 | GLN | THR | LEU | GLY | GLU | ALA | PHE | SER | GLN | PHE | ||||
| 4 | GLY | GLU | ILE | LEU | ASP | SER | LYS | ILE | ILE | ASN | ||||
| 5 | ASP | ARG | GLU | THR | GLY | ARG | SER | ARG | GLY | PHE | ||||
| 6 | GLY | PHE | VAL | THR | PHE | LYS | ASP | GLU | LYS | ALA | ||||
| 7 | MET | ARG | ASP | ALA | ILE | GLU | GLY | MET | ASN | GLY | ||||
| 8 | GLN | ASP | LEU | ASP | GLY | ARG | ASN | ILE | THR | VAL | ||||
| 9 | ASN | GLU | ALA | GLN | SER | ARG |
Samples:
sample_1: RNA-BINDING GLYCINE-RICH PROTEIN, [U-13C; U-15N], 0.4 mM
sample_conditions_1: ionic strength: 150.000 mM; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - chemical shift assignment
ANALYSIS vany, CCPN - chemical shift assignment
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 750 MHz
Related Database Links:
| GB | ADG03637.1 ABH07505 ADG03637 |
| UNP | D6PZY5_TOBAC |
| PDB | |
| DBJ | BAA03741 |
| REF | XP_009774643 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts