BMRB Entry 19531
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19531
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR spatial structure of the trimeric mutant TM domain of VEGFR2 receptor.
Deposition date: 2013-10-02 Original release date: 2014-07-28
Authors: Mineev, Konstantin; Arseniev, Alexander; Shulepko, Mikhail; Lyukmanova, Ekaterina; Kirpichnikov, Mikhail
Citation: Mineev, Konstantin; Arseniev, Alexander; Shulepko, Mikhail; Lyukmanova, Ekaterina; Kirpichnikov, Mikhail. "NMR spatial structure of the trimeric mutant TM domain of VEGFR2 receptor" To be Published ., .-..
Assembly members:
VEGFR-2tm769, polymer, 37 residues, 4161.088 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: E. coli - cell free
Entity Sequences (FASTA):
VEGFR-2tm769: EKTNLEIIILEGTAVIAMFF
WLLLVIILRTVKRANGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 171 |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 310 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VEGFR-2tm769, 1 | 1 |
| 2 | VEGFR-2tm769, 2 | 1 |
| 3 | VEGFR-2tm769, 3 | 1 |
Entities:
Entity 1, VEGFR-2tm769, 1 37 residues - 4161.088 Da.
| 1 | GLU | LYS | THR | ASN | LEU | GLU | ILE | ILE | ILE | LEU | ||||
| 2 | GLU | GLY | THR | ALA | VAL | ILE | ALA | MET | PHE | PHE | ||||
| 3 | TRP | LEU | LEU | LEU | VAL | ILE | ILE | LEU | ARG | THR | ||||
| 4 | VAL | LYS | ARG | ALA | ASN | GLY | GLY |
Samples:
sample_1: VEGFR-2tm769 0.6 mM; VEGFR-2tm769, [U-100% 13C; U-100% 15N], 0.6 mM; DPC, [U-100% 2H], 120 mM; sodium acetate, [U-99% 2H], 20 mM; sodium azide 3 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 20 mM; pH: 4.5; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 13C,15N - filtered NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis
TOPSPIN v3.0, Bruker Biospin - collection
qMDD v2.1, Orekhov, Mayzel - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 19041 |
| PDB | |
| DBJ | BAD93138 |
| GB | AAB34594 AAB88005 AAC16450 AAI31823 EAX05462 |
| PIR | I51296 |
| REF | NP_002244 XP_001086814 XP_002745866 XP_002814831 XP_003268434 |
| SP | P35968 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts