BMRB Entry 19593
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19593
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Title: Truncated EGF-A PubMed: 24440079
Deposition date: 2013-10-30 Original release date: 2014-02-11
Authors: Schroeder, Christina; Rosengren, K. Johan
Citation: Schroeder, Christina; Swedberg, Joakim; Withka, Jane; Rosengren, K. Johan; Akcan, Muharrem; Clayton, Daniel; Daly, Norelle; Cheneval, Olivier; Borzilleri, Kris; Griffor, Matt; Stock, Ingrid; Colless, Barbara; Walsh, Phillip; Sunderland, Philip; Reyes, Allan; Dullea, Robert; Ammirati, Mark; Liu, Shenping; McClure, Kim; Tu, Meihua; Bhattacharya, Samit; Liras, Spiros; Price, David; Craik, David. "Design and synthesis of truncated EGF-A peptides that restore LDL-R recycling in the presence of PCSK9 in vitro." Chem. Biol. 21, 284-294 (2014).
Assembly members:
egfa, polymer, 27 residues, 2774.056 Da.
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
egfa: GTNECLDNNGGCSHVCNDLK
IGYECLX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 62 |
| 1H chemical shifts | 158 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Truncated EGF-A | 1 |
| 2 | CALCIUM ION | 2 |
Entities:
Entity 1, Truncated EGF-A 27 residues - 2774.056 Da.
| 1 | GLY | THR | ASN | GLU | CYS | LEU | ASP | ASN | ASN | GLY | ||||
| 2 | GLY | CYS | SER | HIS | VAL | CYS | ASN | ASP | LEU | LYS | ||||
| 3 | ILE | GLY | TYR | GLU | CYS | LEU | NH2 |
Entity 2, CALCIUM ION - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: egfa 1 mM; d-Tris 50 mM; NaCl 100 mM; CaCl2 5 mM
sample_conditions_1: pH: 5.3; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
Molmol, Koradi, Billeter and Wuthrich - visualisation
TOPSPIN, Bruker Biospin - collection, processing
XEASY, Bartels et al. - chemical shift assignment, peak picking
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92646 BAG10794 BAG58495 BAG59010 BAG61112 |
| GB | AAA56833 AAF24515 AAH14514 AAM56036 AAP36025 |
| REF | NP_000518 NP_001182727 NP_001182728 NP_001182729 NP_001182732 |
| SP | P01130 |