BMRB Entry 19667
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19667
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Title: Solution structure of the carboxyterminal domain of NusG from Mycobacterium tuberculosis
Deposition date: 2013-12-09 Original release date: 2014-01-27
Authors: Strauss, Martin; Schweimer, Kristian; Roesch, Paul
Citation: Strauss, Martin; Schweimer, Kristian; Roesch, Paul. "Solution structure of the carboxyterminal domain of NusG from mycobacterium tuberculosis" Not known ., .-..
Assembly members:
entity, polymer, 62 residues, 6711.688 Da.
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GRPVVEVDYEVGESVTVMDG
PFATLPATISEVNAEQQKLK
VLVSIFGRETPVELTFGQVS
KI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 263 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 446 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | carboxyterminal domain of NusG | 1 |
Entities:
Entity 1, carboxyterminal domain of NusG 62 residues - 6711.688 Da.
| 1 | GLY | ARG | PRO | VAL | VAL | GLU | VAL | ASP | TYR | GLU | ||||
| 2 | VAL | GLY | GLU | SER | VAL | THR | VAL | MET | ASP | GLY | ||||
| 3 | PRO | PHE | ALA | THR | LEU | PRO | ALA | THR | ILE | SER | ||||
| 4 | GLU | VAL | ASN | ALA | GLU | GLN | GLN | LYS | LEU | LYS | ||||
| 5 | VAL | LEU | VAL | SER | ILE | PHE | GLY | ARG | GLU | THR | ||||
| 6 | PRO | VAL | GLU | LEU | THR | PHE | GLY | GLN | VAL | SER | ||||
| 7 | LYS | ILE |
Samples:
sample_1: entity, [U-95% 13C; U-90% 15N], 1.1 mM; HEPES 25 mM; sodium chloride 150 mM
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMRView, Johnson, One Moon Scientific - NMR data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAH24951 BAL64520 BAQ04534 GAA44419 |
| EMBL | CAL70674 CCC25720 CCC42978 CCC63246 CCE36182 |
| GB | AAK44893 ABQ72374 ABR04995 ACT23685 AEB02783 |
| REF | NP_215153 NP_854316 WP_003403288 WP_023349453 WP_023370810 |
| SP | P65590 P9WIU8 P9WIU9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts