BMRB Entry 19697
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19697
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Title: Tv1 PubMed: 24713808
Deposition date: 2013-12-20 Original release date: 2014-05-15
Authors: Bhuiyan, Mohammed; Anand, Prachi; Grigoryan, Alexandre; Holford, Mand; Poget, Sebastien
Citation: Anand, Prachi; Grigoryan, Alexandre; Bhuiyan, Mohammed; Ueberheide, Beatrix; Russell, Victoria; Quinonez, Jose; Moy, Patrick; Chait, Brian; Poget, Sebastien; Holford, Mande. "Sample Limited Characterization of a Novel Disulfide-Rich Venom Peptide Toxin from Terebrid Marine Snail Terebra variegata." PLoS ONE 9, e94122-e94122 (2014).
Assembly members:
Tv1, polymer, 21 residues, 2318.668 Da.
Natural source: Common Name: gastropods Taxonomy ID: 1167544 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Terebra variegata
Experimental source: Production method: chemical synthesis Host organism: na
Entity Sequences (FASTA):
Tv1: TRICCGCYWNGSKDVCSQSC
C
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 107 |
| 13C chemical shifts | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tv1 | 1 |
Entities:
Entity 1, Tv1 21 residues - 2318.668 Da.
| 1 | THR | ARG | ILE | CYS | CYS | GLY | CYS | TYR | TRP | ASN | ||||
| 2 | GLY | SER | LYS | ASP | VAL | CYS | SER | GLN | SER | CYS | ||||
| 3 | CYS |
Samples:
h2o: Tv1 260 uM; H2O 90%; D2O, [U-100% 2H], 10%
d2o: Tv1 260 uM; D2O, [U-100% 2H], 100%
sample_conditions_1: ionic strength: 1 mM; pH: 6.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC aliphatic | d2o | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | d2o | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | d2o | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | h2o | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | h2o | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O, . - refinement
TALOS, Cornilescu, Delaglio and Bax - geometry optimization
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Agilent Uniform NMR System 600 MHz
Related Database Links:
| PDB |