BMRB Entry 19710
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19710
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of CDYL2 chromodomain
Deposition date: 2013-12-28 Original release date: 2014-04-22
Authors: Qin, Su; Houliston, Scott; Arrowsmith, Cheryl; Edwards, Aled; Wu, Hong; Min, Jinrong
Citation: Qin, Su; Houliston, Scott; Arrowsmith, Cheryl; Wu, Hong; Min, Jinrong. "Solution structure of CDYL2 chromodomain" Not known ., .-..
Assembly members:
entity, polymer, 64 residues, 7695.667 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GASGDLYEVERIVDKRKNKK
GKWEYLIRWKGYGSTEDTWE
PEHHLLHCEEFIDEFNGLHM
SKDK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 232 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 366 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CDYL2 chromodomain | 1 |
Entities:
Entity 1, CDYL2 chromodomain 64 residues - 7695.667 Da.
| 1 | GLY | ALA | SER | GLY | ASP | LEU | TYR | GLU | VAL | GLU | ||||
| 2 | ARG | ILE | VAL | ASP | LYS | ARG | LYS | ASN | LYS | LYS | ||||
| 3 | GLY | LYS | TRP | GLU | TYR | LEU | ILE | ARG | TRP | LYS | ||||
| 4 | GLY | TYR | GLY | SER | THR | GLU | ASP | THR | TRP | GLU | ||||
| 5 | PRO | GLU | HIS | HIS | LEU | LEU | HIS | CYS | GLU | GLU | ||||
| 6 | PHE | ILE | ASP | GLU | PHE | ASN | GLY | LEU | HIS | MET | ||||
| 7 | SER | LYS | ASP | LYS |
Samples:
sample_1: TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, data analysis
TALOS, Cornilescu, Delaglio and Bax - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
fmcgui v2.5, Lemak A. - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB29852 BAC04720 BAE24934 BAJ21089 |
| GB | AAH58956 AAH69440 AAI00804 AAI00805 AAI00806 |
| REF | NP_083717 NP_689555 XP_003791470 XP_004392121 XP_004690411 |
| SP | Q8N8U2 Q9D5D8 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts