BMRB Entry 19712
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19712
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Title: Designed Exendin-4 analogues PubMed: 24828921
Deposition date: 2013-12-30 Original release date: 2014-06-02
Authors: Rovo, Petra; Farkas, Viktor; Straner, Pal; Jermendy, Agnes; Hegyi, Orsolya; Toth, Gabor; Perczel, Andras
Citation: Rovo, Petra; Farkas, Viktor; Straner, Pal; Szabo, Maria; Jermendy, Agnes; Hegyi, Orsolya; Toth, Gabor; Perczel, Andras. "Rational Design of -Helix-Stabilized Exendin-4 Analogues." Biochemistry 53, 3540-3552 (2014).
Assembly members:
E19, polymer, 37 residues, 4307.771 Da.
Natural source: Common Name: Gila monster Taxonomy ID: 8554 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Heloderma suspectum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E19: EGTFTSDLSKQMEEEAVRLY
IQWLKEGGPSSGRPPPS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 250 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E19 | 1 |
Entities:
Entity 1, E19 37 residues - 4307.771 Da.
| 1 | GLU | GLY | THR | PHE | THR | SER | ASP | LEU | SER | LYS | ||||
| 2 | GLN | MET | GLU | GLU | GLU | ALA | VAL | ARG | LEU | TYR | ||||
| 3 | ILE | GLN | TRP | LEU | LYS | GLU | GLY | GLY | PRO | SER | ||||
| 4 | SER | GLY | ARG | PRO | PRO | PRO | SER |
Samples:
sample: E19 0.5-1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 300 K; viscosity: 1.002 cP
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | sample | isotropic | sample_conditions_1 |
| NOESY | sample | isotropic | sample_conditions_1 |
| COSY | sample | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
NMR spectrometers:
- Bruker DRX 700 MHz
Related Database Links:
| PDB |