BMRB Entry 19716
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19716
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Title: Zn-binding domain of eukaryotic translation initiation factor 3, subunit G
Deposition date: 2014-01-03 Original release date: 2015-01-05
Authors: Al-Abdul-Wahid, Mohamed Sameer; Menade, Marie; Xie, Jingwei; Kozlov, Guennadi; Gehring, Kalle
Citation: Al-Abdul-Wahid, Mohamed Sameer; Menade, Marie; Xie, Jingwei; Kozlov, Guennadi; Gehring, Kalle. "Solution NMR structure of the Zn-binding domain of eukaryotic translation initiation factor 3, subunit G" Proteins: Struct. Funct. Genet. ., .-..
Assembly members:
eif3G_Zn, polymer, 24 residues, 2787.301 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
eif3G_Zn: KIVSCRICKGDHWTTRCPYK
DTLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 78 |
| 15N chemical shifts | 24 |
| 1H chemical shifts | 152 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, entity_1 24 residues - 2787.301 Da.
| 1 | LYS | ILE | VAL | SER | CYS | ARG | ILE | CYS | LYS | GLY | ||||
| 2 | ASP | HIS | TRP | THR | THR | ARG | CYS | PRO | TYR | LYS | ||||
| 3 | ASP | THR | LEU | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: eif3G_Zn 10 mM; HEPES 10 mM; sodium chloride 50 mM; zinc chloride 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
XPLOR-NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAC29952 BAE40297 BAE87500 BAF84009 BAH11496 |
| EMBL | CAG12187 CAG33415 CAJ83120 CDQ73632 CDQ81098 |
| GB | AAB71866 AAC78728 AAD00176 AAF14221 AAG15396 |
| REF | NP_001013113 NP_001016749 NP_001073091 NP_001086477 NP_001087888 |
| SP | O75821 Q28CY2 Q3ZC12 Q5RK09 Q641B2 |
| TPG | DAA27948 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts