BMRB Entry 19766

Name: Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex
Published: 2014-08-27

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PDB ID: 2mkc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19766
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

Deposition date: 2014-02-04 Original release date: 2014-08-27

Authors: Wysoczanski, Piotr; Schneider, Cornelius; Xiang, ShengQi; Munari, Francesca; Trowitzsch, Simon; Wahl, Markus; Luhrmann, Reinhard; Becker, Stefan; Zweckstetter, Markus

Citation: Wysoczanski, Piotr; Schneider, Cornelius; Xiang, ShengQi; Munari, Francesca; Trowitzsch, Simon; Wahl, Markus; Luhrmann, Reinhard; Becker, Stefan; Zweckstetter, Markus. "Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex" Nat. Struct. Mol. Biol. ., .-..

Assembly members:
Snu17p, polymer, 118 residues, 13496.103 Da.
Pml1p, polymer, 23 residues, 2514.800 Da.
Bud13p, polymer, 33 residues, 3828.226 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Snu17p: GAMGNEYKDNAYIYIGNLNR ELTEGDILTVFSEYGVPVDV ILSRDENTGESQGFAYLKYE DQRSTILAVDNLNGFKIGGR ALKIDHTFYRPKRSLQKYYE AVKEELDRDIVSKNNAEK
Pml1p: GSKSQYIDIMPDFSPSGLLE LES
Bud13p: GSYDKPAPENRFAIMPGSRW DGVHRSNGFEEKW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	705
15N chemical shifts	180
1H chemical shifts	1188

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	Snu17p	1
2	Pml1p	2
3	Bud13p	3

Samples:

Snu17p_13C15N: sodium phosphate 25 mM; NaCl 250 mM; sodium azide 1 mM; Snu17p, [U-13C; U-15N], 0.9 – 1.3 mM; Bud13p1.1 – 1.5 mM; Pml1p1.4 – 19 mM; H2O 90%; D2O 10%

Pml1p_13C15N: sodium phosphate 25 mM; NaCl 250 mM; sodium azide 1 mM; Snu17p1.0 – 1.3 mM; Bud13p1.2 – 1.5 mM; Pml1p, [U-13C; U-15N], 0.8 – 1.0 mM; H2O 90%; D2O 10%

Bud13p_13C15N: sodium phosphate 25 mM; NaCl 250 mM; sodium azide 1 mM; Snu17p1.0 – 1.3 mM; Bud13p, [U-10% 13C; U-99% 15N], 0.8 – 1.0 mM; Pml1p1.5 – 1.9 mM; H2O 90%; D2O 10%

Snu17p_2H13C15N: sodium phosphate 25 mM; NaCl 250 mM; sodium azide 1 mM; Snu17p, [U-13C; U-15N; U-2H], 0.9 – 1.3 mM; Bud13p1.1 – 1.5 mM; Pml1p1.4 – 1.9 mM; H2O 90%; D2O 10%

Bud13p_13C15N_Snu17p_2H: sodium phosphate 25 mM; NaCl 250 mM; sodium azide 1 mM; Snu17p, [U-13C; U-15N; U-2H], 1.0 – 1.3 mM; Bud13p, [U-10% 13C; U-99% 15N], 0.8 – 1.0 mM; Pml1p1.5 – 1.9 mM; H2O 90%; D2O 10%

CONDITIONS: pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Snu17p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Snu17p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Snu17p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Snu17p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Pml1p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Pml1p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Pml1p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Pml1p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Bud13p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Bud13p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Bud13p_13C15N	isotropic	CONDITIONS
2D 1H-15N HSQC	Bud13p_13C15N	isotropic	CONDITIONS
3D HNCO	Snu17p_13C15N	isotropic	CONDITIONS
3D HNCA	Snu17p_13C15N	isotropic	CONDITIONS
3D HNCA	Pml1p_13C15N	isotropic	CONDITIONS
3D HNCA	Bud13p_13C15N	isotropic	CONDITIONS
3D HNCACB	Snu17p_2H13C15N	isotropic	CONDITIONS
3D HN(CO)CA	Snu17p_13C15N	isotropic	CONDITIONS
3D HN(CO)CA	Pml1p_13C15N	isotropic	CONDITIONS
3D HN(CO)CA	Bud13p_13C15N	isotropic	CONDITIONS
3D HCCH-TOCSY	Snu17p_13C15N	isotropic	CONDITIONS
3D HCCH-TOCSY	Pml1p_13C15N	isotropic	CONDITIONS
3D HCCH-TOCSY	Bud13p_13C15N	isotropic	CONDITIONS
3D 1H-15N NOESY	Snu17p_13C15N	isotropic	CONDITIONS
3D 1H-15N NOESY	Pml1p_13C15N	isotropic	CONDITIONS
3D 1H-15N NOESY	Bud13p_13C15N	isotropic	CONDITIONS
3D 1H-15N NOESY	Bud13p_13C15N_Snu17p_2H	isotropic	CONDITIONS
3D 1H-13C NOESY aliphatic	Snu17p_13C15N	isotropic	CONDITIONS
3D 1H-13C NOESY aliphatic	Pml1p_13C15N	isotropic	CONDITIONS
3D 1H-13C NOESY aliphatic	Bud13p_13C15N	isotropic	CONDITIONS
3D 1H-13C NOESY aliphatic	Bud13p_13C15N_Snu17p_2H	isotropic	CONDITIONS
3D 1H-13C NOESY aromatic	Snu17p_13C15N	isotropic	CONDITIONS
3D 1H-13C NOESY aromatic	Pml1p_13C15N	isotropic	CONDITIONS
3D 1H-13C NOESY aromatic	Bud13p_13C15N	isotropic	CONDITIONS
3D HCCH-TOCSY	Bud13p_13C15N_Snu17p_2H	isotropic	CONDITIONS
3D 1H-13C NOESY filtered/edited	Snu17p_13C15N	isotropic	CONDITIONS
3D 1H-13C NOESY filtered/edited	Pml1p_13C15N	isotropic	CONDITIONS
HBCBCGCDHD	Snu17p_13C15N	isotropic	CONDITIONS
3D CCH-TOCSY	Snu17p_13C15N	isotropic	CONDITIONS
3D CBCA(CO)NH	Bud13p_13C15N	isotropic	CONDITIONS
3D 1H-15N NOESY ARG-centered	Bud13p_13C15N	isotropic	CONDITIONS

UNP	P40565 Q07930 P46947
BMRB	25047
PDB	2MKC 4UQT 2JKD 2MKC 3ELV 2MKC
DBJ	GAA24120 GAA24903 GAA23224
EMBL	CAA86207 CAY80517 CAA62157 CAA97538 CAY81254 CAA59179 CAA96886 CAY79593
GB	AAS56407 AEO21093 AHY76002 AJP39470 AJR36763 AAS56260 AHY78422 EDN59562 EDV09324 EDZ70737 AAS56830 AHY79214 EDN61950 EDV10384 EDZ72293
REF	NP_012270 NP_013116 NP_011341
SP	P40565 Q07930 P46947
TPG	DAA08551 DAA09334 DAA07939

Biological Magnetic Resonance Data Bank

BMRB Entry 19766

Title: Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

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