BMRB Entry 19773
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19773
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Title: Backbone chemical shifts for the E81 deletion mutant from RAP80 tandem UIMs PubMed: 24627472
Deposition date: 2014-02-06 Original release date: 2014-02-06
Authors: Anamika, Anamika; Markin, Craig; Rout, Manoj; Spyracopoulos, Leo
Citation: Anamika, Anamika; Markin, Craig; Rout, Manoj; Spyracopoulos, Leo. "Molecular Basis for Impaired DNA Damage Response Function Associated with the RAP80 E81 Defect." J. Biol. Chem. ., .-. (2014).
Assembly members:
entity, polymer, 62 residues, 6889.636 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GPLGSRKIAQMTEEQFALAL
KMSEQEAREVNSQEEEEEEL
LRKAIAESLNSCRPSDASAT
RS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 113 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 114 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 62 residues - 6889.636 Da.
| 1 | GLY | PRO | LEU | GLY | SER | ARG | LYS | ILE | ALA | GLN | ||||
| 2 | MET | THR | GLU | GLU | GLN | PHE | ALA | LEU | ALA | LEU | ||||
| 3 | LYS | MET | SER | GLU | GLN | GLU | ALA | ARG | GLU | VAL | ||||
| 4 | ASN | SER | GLN | GLU | GLU | GLU | GLU | GLU | GLU | LEU | ||||
| 5 | LEU | ARG | LYS | ALA | ILE | ALA | GLU | SER | LEU | ASN | ||||
| 6 | SER | CYS | ARG | PRO | SER | ASP | ALA | SER | ALA | THR | ||||
| 7 | ARG | SER |
Samples:
sample_1: deltaE81-RAP80-tUIM, [U-100% 15N], .5 – 1 mM; TRIS 50 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_2: deltaE81-RAP80-tUIM, [U-100% 13C; U-100% 15N], .5 – 1 mM; TRIS 50 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K
sample_conditions_2: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_2 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_2 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_2 |
| 3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_2 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 19774 |
| PDB | |
| GB | AAG59851 ERE80156 |
| REF | XP_006220429 XP_007124893 XP_008013593 XP_008988510 XP_012516199 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts