BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 19806

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19806
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Title: NMR structure of hypothetical protein ZP_02064002.1 from Bacteroides ovatus ATCC 8483

Deposition date: 2014-02-19 Original release date: 2015-09-02

Authors: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of hypothetical protein ZP_02064002.1 from Bacteroides ovatus ATCC 8483"  Not known ., .-..

Assembly members:
entity, polymer, 155 residues, Formula weight is not available

Natural source:   Common Name: CFB Group Bacteria   Taxonomy ID: 411476   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacteroides ovatus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GDSELTTQDGEDFKSFLDKF TSSAAFQYTRVKFPLKTPIT LLADDGETEKTFPFTKEKWP LLDSETMKEERITQEEGGIY VSKFTLNEPKHKIFEAGYEE SEVDLRVEFELQADGKWYVV DCYTGWYGYDLPIGELKQTI QNVKEENAAFKEIHP

Data sets:
Data typeCount
13C chemical shifts497
15N chemical shifts158
1H chemical shifts1042

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 155 residues - Formula weight is not available

1   GLYASPSERGLULEUTHRTHRGLNASPGLY
2   GLUASPPHELYSSERPHELEUASPLYSPHE
3   THRSERSERALAALAPHEGLNTYRTHRARG
4   VALLYSPHEPROLEULYSTHRPROILETHR
5   LEULEUALAASPASPGLYGLUTHRGLULYS
6   THRPHEPROPHETHRLYSGLULYSTRPPRO
7   LEULEUASPSERGLUTHRMETLYSGLUGLU
8   ARGILETHRGLNGLUGLUGLYGLYILETYR
9   VALSERLYSPHETHRLEUASNGLUPROLYS
10   HISLYSILEPHEGLUALAGLYTYRGLUGLU
11   SERGLUVALASPLEUARGVALGLUPHEGLU
12   LEUGLNALAASPGLYLYSTRPTYRVALVAL
13   ASPCYSTYRTHRGLYTRPTYRGLYTYRASP
14   LEUPROILEGLYGLULEULYSGLNTHRILE
15   GLNASNVALLYSGLUGLUASNALAALAPHE
16   LYSGLUILEHISPRO

Samples:

sample_1: entity, [U-99% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.0798 M; pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

CYANA, G ntert P., Herrmann and Wuthrich - chemical shift assignment, peak picking, structure solution

CYANA, G ntert P., Herrmann and Wuthrich - chemical shift assignment, peak picking, structure solution

CARA, Keller and Wuthrich - data analysis

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - geometry optimization

TOPSPIN v3.1, Bruker Biospin - collection, processing

PROSA, Guntert - processing

GAPRO, Hiller S - peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CBK67627 CDM02213 CDM07816 CUO57325
GB ALJ48042 EDO13427 EEO48558 EEO56081 EEZ05524
REF WP_004298133 WP_004313843 WP_008649001 WP_032811951 WP_032852245

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts