BMRB Entry 19824
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19824
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Title: Solution structure of a TrkAIg2 domain construct for use in drug discovery
Deposition date: 2014-02-28 Original release date: 2015-01-12
Authors: Shoemark, D.; Fahey, M.; Williams, C.; Sessions, R.; Crump, Matthew; Allen-birt, S.; A., S.
Citation: Allen, S.; Watson, J.; Shoemark, D.; Barua, N.; Patel, N.. "GDNF, NGF and BDNF as therapeutic options for neurodegeneration." Pharmacology & Therapeutics ., .-..
Assembly members:
HIGH_AFFINITY_NERVE_GROWTH_FACTOR_RECEPTOR, polymer, 107 residues, 11776.0322 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HIGH_AFFINITY_NERVE_GROWTH_FACTOR_RECEPTOR: DDDDDVSFCASVQLHTAVEM
HHWCIPFSVDGQPAPSLRWL
FNGSVLNETSFIFTEFLEPA
ANETVRHGCLRLNQPTHVNN
GNYTLLAANPCGQASASIMA
AFMDNPF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 441 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 690 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIGH AFFINITY NERVE GROWTH FACTOR RECEPTOR | 1 |
Entities:
Entity 1, HIGH AFFINITY NERVE GROWTH FACTOR RECEPTOR 107 residues - 11776.0322 Da.
| 1 | ASP | ASP | ASP | ASP | ASP | VAL | SER | PHE | CYS | ALA | ||||
| 2 | SER | VAL | GLN | LEU | HIS | THR | ALA | VAL | GLU | MET | ||||
| 3 | HIS | HIS | TRP | CYS | ILE | PRO | PHE | SER | VAL | ASP | ||||
| 4 | GLY | GLN | PRO | ALA | PRO | SER | LEU | ARG | TRP | LEU | ||||
| 5 | PHE | ASN | GLY | SER | VAL | LEU | ASN | GLU | THR | SER | ||||
| 6 | PHE | ILE | PHE | THR | GLU | PHE | LEU | GLU | PRO | ALA | ||||
| 7 | ALA | ASN | GLU | THR | VAL | ARG | HIS | GLY | CYS | LEU | ||||
| 8 | ARG | LEU | ASN | GLN | PRO | THR | HIS | VAL | ASN | ASN | ||||
| 9 | GLY | ASN | TYR | THR | LEU | LEU | ALA | ALA | ASN | PRO | ||||
| 10 | CYS | GLY | GLN | ALA | SER | ALA | SER | ILE | MET | ALA | ||||
| 11 | ALA | PHE | MET | ASP | ASN | PRO | PHE |
Samples:
sample_1: HIGH AFFINITY NERVE GROWTH FACTOR RECEPTOR, [U-13C; U-15N], 0.75 ± 0.25 mM
sample_conditions_1: ionic strength: 10.000 mM; pH: 6.900; pressure: 1.000 atm; temperature: 293.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | solution | sample_conditions_1 |
| TOCSY | sample_1 | solution | sample_conditions_1 |
| HNCA | sample_1 | solution | sample_conditions_1 |
| HNCACB | sample_1 | solution | sample_conditions_1 |
| CCONH | sample_1 | solution | sample_conditions_1 |
| HCCH-TOCSY | sample_1 | solution | sample_conditions_1 |
| HCCONH | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, data analysis
NMR spectrometers:
- Varian UnityInova 600 MHz
Related Database Links:
| UNP | NTRK1_HUMAN |
| PDB | |
| DBJ | BAA34355 BAF83448 BAG35582 |
| GB | AAH62580 AAI36555 AAI44240 AHW56542 AHW56543 |
| REF | NP_001007793 NP_001012331 NP_002520 XP_001114243 XP_001145942 |
| SP | P04629 |
Download simulated HSQC data in one of the following formats:
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