BMRB Entry 19848
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19848
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR solution structure of PA3793 from Pseudomonas aeruginosa
Deposition date: 2014-03-11 Original release date: 2014-04-28
Authors: Mayzel, M.; Schnell, R.; Schneider, G.; Karlsson, Goran
Citation: Schneider, G.; Schnell, R.; Mayzel, M.; Karlsson, Goran. "NMR SOLUTION STRUCTURE OF PA3793 FROM PSEUDOMONAS" Not known ., .-..
Assembly members:
UNCHARACTERIZED_PROTEIN, polymer, 113 residues, 12725.7071 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: E. coli
Entity Sequences (FASTA):
UNCHARACTERIZED_PROTEIN: GSHMTTQRYLLDELETADML
EIDGLHAWRFELNENLLDQA
DLAAEADQPFASEDWVLAVE
SLDGRTRREWRFSYNAVMEA
EPQADGESWRLTTGEGAYQL
RCLGAVSASGEDE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 462 |
| 15N chemical shifts | 120 |
| 1H chemical shifts | 729 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PA3793 from Pseudomonas aeruginosa | 1 |
Entities:
Entity 1, PA3793 from Pseudomonas aeruginosa 113 residues - 12725.7071 Da.
| 1 | GLY | SER | HIS | MET | THR | THR | GLN | ARG | TYR | LEU | ||||
| 2 | LEU | ASP | GLU | LEU | GLU | THR | ALA | ASP | MET | LEU | ||||
| 3 | GLU | ILE | ASP | GLY | LEU | HIS | ALA | TRP | ARG | PHE | ||||
| 4 | GLU | LEU | ASN | GLU | ASN | LEU | LEU | ASP | GLN | ALA | ||||
| 5 | ASP | LEU | ALA | ALA | GLU | ALA | ASP | GLN | PRO | PHE | ||||
| 6 | ALA | SER | GLU | ASP | TRP | VAL | LEU | ALA | VAL | GLU | ||||
| 7 | SER | LEU | ASP | GLY | ARG | THR | ARG | ARG | GLU | TRP | ||||
| 8 | ARG | PHE | SER | TYR | ASN | ALA | VAL | MET | GLU | ALA | ||||
| 9 | GLU | PRO | GLN | ALA | ASP | GLY | GLU | SER | TRP | ARG | ||||
| 10 | LEU | THR | THR | GLY | GLU | GLY | ALA | TYR | GLN | LEU | ||||
| 11 | ARG | CYS | LEU | GLY | ALA | VAL | SER | ALA | SER | GLY | ||||
| 12 | GLU | ASP | GLU |
Samples:
sample_1: UNCHARACTERIZED PROTEIN, [U-13C; U-15N], 1.6 mM
sample_2: UNCHARACTERIZED PROTEIN, [U-13C; U-15N], 1.6 mM
sample_3: UNCHARACTERIZED PROTEIN 1.6 mM
sample_conditions_1: ionic strength: 70.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15Nnoesy | sample_1 | solution | sample_conditions_1 |
| 15Nhsqc | sample_1 | solution | sample_conditions_1 |
| backbone exps | sample_1 | solution | sample_conditions_1 |
| 13C noesy | sample_2 | solution | sample_conditions_1 |
| 2D noesy | sample_3 | solution | sample_conditions_1 |
| 2D tocsy | sample_3 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, Sen et al. - collection
Analysis vany, CCPN - chemical shift assignment
CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
NMR spectrometers:
- Varian UnityInova 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts